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Nucleotide Binding to the Human Multidrug Resistance Protein 3, MRP3

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Abstract

We used a spin-labeled ATP analog, SL-ATP, to study nucleotide binding to highly purified human multidrug resistance protein 3, MRP3, which had been expressed in the yeast Pichia pastoris. SL-ATP was shown to be a good substrate analog and is hydrolyzed by MRP3 at about 10% of the Vmax for normal ATP. ESR titrations showed that 2 mol of SL-ATP readily bound per mole of MRP3 with a dissociation constant of about 100 μM in the presence of Mg2+ ions. The binding curve was easily fitted for a hyperbolic binding relationship. SL-ATP also bound readily to MRP3 in the absence of divalent ions and presence of EDTA. The resulting binding curve, however, could not be satisfactorily fitted using the equation for hyperbola. Analysis showed that a good fit was only obtained with the Hill equation using a Hill coefficient of 4 or close to 4. Lower Hill coefficients resulted in lower goodness of the fit. Such cooperative binding may be explained by a dimerization event triggered in the absence of divalent ions and a close communication of nucleotide binding sites of the interacting dimers. These findings may be of great importance for the overall mechanism and regulation of multidrug resistance proteins.

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Acknowledgments

This work was supported by a grant from the SMU University Research Council to PDV and a grant from the Jasper and Jack Wilson Foundation to ILU. The authors wish to thank J. G. Wise for helpful discussions. The authors wish to thank Dr. Jürgen Zirkel, Lipoid GmbH, Germany, for providing us with 100% pure lyso-phosphatidyl cholin.

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  1. Andrea D. Hoffman

    Present address: Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA

Authors and Affiliations

  1. Department of Biological Sciences, Southern Methodist University, Dallas, TX, 75275, USA

    Andrea D. Hoffman & Pia D. Vogel

  2. Cell Biology and Biochemistry, Texas Tech University Health Science Center, Lubbock, TX, 79430, USA

    Ina L. Urbatsch

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  1. Andrea D. Hoffman
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  2. Ina L. Urbatsch
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  3. Pia D. Vogel
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Correspondence to Pia D. Vogel.

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Hoffman, A.D., Urbatsch, I.L. & Vogel, P.D. Nucleotide Binding to the Human Multidrug Resistance Protein 3, MRP3. Protein J 29, 373–379 (2010). https://doi.org/10.1007/s10930-010-9262-4

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