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Purification and Characterization of a Proteinaceous Toxin from the Serum of Japanese Eel Anguilla japonica

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Abstract

Although eels are well known to contain toxins in the serum, their chemical properties have remained to be clarified for a long time. In this study, a proteinaceous toxin was purified from the serum of Japanese eel Anguilla japonica by anion-exchange HPLC, hydroxyapatite HPLC and gel filtration HPLC. The toxin was lethal to both mice and crabs; the LD50 of the purified toxin against mice (intravenous injection) and crabs (injection into body cavity) were estimated to be 670 and 450 μg kg−1, respectively. Chemical analysis data revealed that the toxin is a monomeric simple protein with a molecular mass of 100 kDa and an isoelectric point of 6.1. Three of the peptide fragments produced by digestion of the purified toxin with lysylendopeptidase were sequenced. However, a database search based on the determined partial amino acid sequence failed to find any proteins sharing homology with the A. japonica serum toxin.

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Abbreviations

HPLC:

High performance liquid chromatography

LD50 :

50% Lethal dose

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo, 108-8477, Japan

    Mireiyu Yoshida, Seiji Sone & Kazuo Shiomi

Authors
  1. Mireiyu Yoshida
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  2. Seiji Sone
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  3. Kazuo Shiomi
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Corresponding author

Correspondence to Kazuo Shiomi.

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Yoshida, M., Sone, S. & Shiomi, K. Purification and Characterization of a Proteinaceous Toxin from the Serum of Japanese Eel Anguilla japonica . Protein J 27, 450–454 (2008). https://doi.org/10.1007/s10930-008-9155-y

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  • DOI: https://doi.org/10.1007/s10930-008-9155-y

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