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Isolation and Characterization of Low-Molecular-Weight Chromium-binding Substance (LMWCr) from Chicken Liver

Abstract

Chromodulin (also known as low-molecular-weight chromium-binding substance, LMWCr) is a chromium-binding oligopeptide proposed to play a role in insulin signaling and chromium transport in mammals. This laboratory has isolated and purified this material from a non-mammalian source, an avian. Spectroscopic and physical characterization of the isolated material suggests the material is an oligopeptide with a multinuclear chromium assembly bridged via asparatate and glutamate residues very similar to its mammalian counterparts. The isolated material also possesses a biological activity similar to other LMWCr isolates.

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Abbreviations

LMWCr:

Low-molecular-weight chromium-binding substance

ADAM 19:

A disintegrin and metalloproteinase domain 19

Glu:

Glutamate

Asp:

Aspartate

mL:

Milliliter

M:

Molar

mM:

Millimolar

μM:

Micomolar

g :

Gravity

ºC:

Degree celsius

%:

Percent

pH:

Measure of acidity or basicity

cm:

Centimeter

nm:

Nanometer

μL:

Microliter

ppm:

Parts per million

DEAE:

(Diethylaminoethyl) cellulose

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

1H NMR:

Proton nuclear magnetic resonance

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Acknowledgements

The Department of Chemistry at Georgia Southern University in conjugation with the Allen E. Paulson College of Science and Technology Academic Excellence Committee provided funding for this research. The authors would like to acknowledge and thank Phoebe C. Pletcher for her technical assistance in the chromium and protein assays.

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Correspondence to C. Michele Davis-McGibony.

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Viera, M., Davis-McGibony, C.M. Isolation and Characterization of Low-Molecular-Weight Chromium-binding Substance (LMWCr) from Chicken Liver. Protein J 27, 371–375 (2008). https://doi.org/10.1007/s10930-008-9146-z

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Keywords

  • Chromium
  • Chicken
  • Low-molecular-weight chromium-binding substance
  • Chromodulin