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Contribution of the Conserved A16Leu to Insulin Foldability

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Abstract

Contributions of the evolutionarily conserved A16Leu and B17Leu to insulin foldability were characterized by evaluating folding properties of single-chain insulin analogs. The results showed A16Leu had much more significant effects on the foldability of insulin than B17Leu.

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Abbreviations

PIP:

Porcine insulin precursor in which B30Ala and A1Gly of porcine insulin are linked together by a dipeptide, Ala-Lys

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

EDTA:

Ethylenediaminetetraacetic acid

HPLC:

High performance liquid chromatography

TFA:

Trifluoroacetic acid

PAGE:

Polyacrylamide gel electrophoresis

UV:

Ultraviolet

CD:

Circular dichroism

NMR:

Nuclear magnetic resonance

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Authors and Affiliations

  1. Institute of Molecular Biology, College of Life Sciences, Nan Kai University, Tian Jin, 300071, China

    Zhao-Jun Zhang & Ming-Qiang Qiao

  2. Department of Horticulture, Beijing Vocational College of Agriculture, Beijing, 102442, China

    Lan Wu

  3. Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Zhi-Song Qiao & You-Min Feng

  4. Institute of Protein Research, Tongji University, Shanghai, 200092, China

    Zhan-Yun Guo

Authors
  1. Zhao-Jun Zhang
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  2. Lan Wu
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  3. Zhi-Song Qiao
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  4. Ming-Qiang Qiao
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  5. You-Min Feng
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  6. Zhan-Yun Guo
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Corresponding authors

Correspondence to You-Min Feng or Zhan-Yun Guo.

Additional information

Zhao-Jun Zhang and Lan Wu have equally contributed to this work.

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Zhang, ZJ., Wu, L., Qiao, ZS. et al. Contribution of the Conserved A16Leu to Insulin Foldability. Protein J 27, 192–196 (2008). https://doi.org/10.1007/s10930-007-9124-x

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  • DOI: https://doi.org/10.1007/s10930-007-9124-x

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