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Development and Characterization of Graphene Oxide-Locust Bean Gum-Zinc Oxide (GO-LBG-ZnO) Nanohybrid as an Efficient and Novel Antitumor Agent against Hepatocarcinoma Cells

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Abstract

The main goal of this study was to introduce a nanohybrid based on graphene oxide and zinc oxide with strong antitumor activity for the treatment of hepatitis B virus-associated hepatocarcinoma. In this regard, the nanohybrid was fabricated through the first attachment of locust bean gum chains on the graphene oxide surface and subsequently zinc oxide nanoparticles decorated on the surface of graphene oxide-locust bean gum nanosheets. Then, the prepared ternary nanohybrid was assessed in terms of physicochemical and microstructural characteristics, cytotoxicity, and antitumor activity. Our primary results indicated the successful fabrication of nanohybrids. The results of toxicity assays showed that the prepared nanohybrid represented higher cytotoxicity on the cancer cell line compared to the normal cells. Antitumor activity results demonstrated that the synthesized nanohybrid can superiorly decline viral and cellular oncogenes and induce cell apoptosis in liver cancer cells. In conclusion, the fabricated nanohybrid demonstrated strong antitumor activity against hepatocarcinoma cells, which can be considered a promising candidate for the treatment of hepatocarcinoma.

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Acknowledgements

The Ethical Committee of Kerman University of Medical Sciences (IR.KMU.AH.REC.1400.223 with grant number 400000202) approved the study.

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  1. Samaneh Shakibaee and Hoomaan Joze-Majidi equally contributed to this work.

Authors and Affiliations

  1. Department of Medical Microbiology (Bacteriology and Virology), Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

    Samane Shakibaie, Saeedeh Ebrahimi & Elham Mousavi

  2. Department of Polymer Engineering, Faculty of Engineering, Golestan University, Gorgan, Iran

    Hoomaan Joze-Majidi & Erfan Zabihi

  3. Department of Genetics, Golestan University of Medical Sciences, Gorgan, Iran

    Mahboobeh Ramezani

  4. Medical Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran

    Mahboobeh Ramezani

  5. Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran

    Zahra Arab-Bafrani

  6. Department of Biochemistry and Biophysics, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran

    Zahra Arab-Bafrani

  7. Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Elham Mousavi

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  1. Samane Shakibaie
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Contributions

M.E and A-B.Z designed the research. Sh.S, J-M.H, Z.E, R.M and M.E performed the experiments. A-B.Z, M.E and E.S contributed to analysis and interpretation of data. J-M.H, A-B.Z and M.E wrote the manuscript. All authors reviewed the manuscript.

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Correspondence to Zahra Arab-Bafrani or Elham Mousavi.

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Shakibaie, S., Joze-Majidi, H., Zabihi, E. et al. Development and Characterization of Graphene Oxide-Locust Bean Gum-Zinc Oxide (GO-LBG-ZnO) Nanohybrid as an Efficient and Novel Antitumor Agent against Hepatocarcinoma Cells. J Polym Environ (2024). https://doi.org/10.1007/s10924-023-03184-3

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