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Freeze-Dried Carboxymethyl Chitosan/Starch Foam for Use as a Haemostatic Wound Dressing

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Abstract

Prolonged bleeding is a general complication that occurs after tooth extraction or oral surgery. Thus, patients must apply pressure and use absorbable wound dressings to stop bleeding and prevent blood loss. This method stops bleeding and causes clotting of blood. However, some people have bleeding disorders or cannot stop bleeding with applied pressure after oral surgery. Therefore, haemostatic foam dressing is an interesting material that can be used to stop bleeding without hindering blood clotting in the wound site. In this study, foam was prepared using a blend of starch with carboxymethyl chitosan and glyoxal as a crosslinking agent. The foam was formed by freeze-drying. Carboxymethyl chitosan/starch foam absorbed water rapidly and held it, as analysed by a blood clotting assay and maximum swelling. Thus, carboxymethyl chitosan/starch foam (CM/starch foam) made with a 1:4 ratio and with 1% glyoxal also absorbed blood very well and exhibited suitable properties. Cytotoxic studies using human dermal fibroblast cells, as determined by MTT assay, confirmed that CM/starch foam was non-toxic and had good mechanical properties in a wet environment.

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Acknowledgements

The authors acknowledge financial support received from the Research Pyramid, Rachadaphiseksomphot Endowment Fund (GCURP_58_02_63_01) of Chulalongkorn University. This work was supported in part by (1) the Petroleum and Petrochemical College, Chulalongkorn University, (2) the 90th Anniversary of Chulalongkorn University Fund (Rachadaphiseksomphot Endowment Fund) (3) the Center of Excellence for Petroleum, Petrochemicals, and Advanced Materials (CE-PPAM) (4) The Royal Government of Thailand Scholarship 2562 and (5) Herbal Extracts-infused Advanced Wound Dressing Research Unit, Rachadaphiseksomphot Endowment Fund, Chulalongkorn University.

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Authors and Affiliations

  1. The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, 10330, Thailand

    Nalintorn Jungprasertchai, Pongpol Ekabutr & Pitt Supaphol

  2. Laboratory of Organic Synthesis, Chulabhorn Research Institute, Bangkok, 10210, Thailand

    Piyachat Chuysinuan

  3. Department of Materials Engineering, Faculty of Engineering, Rajamangala University of Technology Rattanakosin, 96 Mu 3 Phutthamonthon Sai 5 Road, Salaya, Phutthamonthon, Nakorn Pathom, 73170, Thailand

    Pimolpun Niamlang

  4. Research Unit on Herbal Extracts-Infused Advanced Wound Dressing, Chulalongkorn University, Bangkok, 10330, Thailand

    Pitt Supaphol

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  1. Nalintorn Jungprasertchai
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  2. Piyachat Chuysinuan
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  3. Pongpol Ekabutr
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Correspondence to Pitt Supaphol.

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Jungprasertchai, N., Chuysinuan, P., Ekabutr, P. et al. Freeze-Dried Carboxymethyl Chitosan/Starch Foam for Use as a Haemostatic Wound Dressing. J Polym Environ 30, 1106–1117 (2022). https://doi.org/10.1007/s10924-021-02260-w

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  • DOI: https://doi.org/10.1007/s10924-021-02260-w

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