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Chitosan in Eucalyptus grandis Pyroligneous Liquor for Agricultural Application: Physicochemical and Structural Characterization During Storage

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Abstract

Pyroligneous liquor and chitosan are natural and biodegradable products from renewable sources. Both present antimicrobial and antioxidant activities and induce resistance in plants, making these products promising to be used as raw material for agricultural inputs. Chitosan, dissolved in distilled pyroligneous liquor (DPL) from Eucalyptus grandis, produces colloidal systems with high ultraviolet absorption and it could be proposed as plant protective coating, based on their intrinsic characteristics. Chitosan undergoes degradation in an acidic environment; therefore, it is necessary to understand the stability of colloidal systems. We evaluated the systems obtained from chitosan and DPL for their physicochemical properties and structural characteristics at storage times at room temperature (20 ± 5 °C) up to 30 months. The colloidal systems presented a Newtonian flow during the storage time evaluated. Posteriorly, the colloidal systems were destabilized and the precipitates (PPT) were analyzed. Phenolic compounds from DPL were attached to the chitosan chains; this result is supported by fluorescence spectroscopy performed with PPT dissolved in non-fluorescent buffer. The PPT were fluorescent with emission maxima changing to higher wavelengths with storage time. The amounts of total phenols attached to polymer chains increased over time. As a result, there is an increase in the absorption of ultraviolet radiation with storage time. The results show that colloidal systems composed by chitosan formed complexes with phenolic compounds from DPL, which are promising for application in agriculture.

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Acknowledgements

The authors thank to Empresa Brasileira de Pesquisa Agropecuária, specially to Laboratório de Fisiologia Vegetal da Embrapa Clima Temperado, Conselho Nacional de Pesquisa (CNPq) (Proc. 311736/2015-7) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ( CAPES 001) for financial support, CNANO for infrared and NMR analyses, and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS).

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Authors and Affiliations

  1. Programa de Pós Graduação em Ciência e Engenharia de Materiais, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rua Gomes Carneiro nº1, Pelotas, 96010-610, Brazil

    Fabiane Grecco da Silva Porto & Neftalí Lenin Villarreal Carreño

  2. Programa de Pós-Graduação em Química, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Porto Alegre, 91501-970, Brazil

    Eduardo dos Santos Vasconcelos

  3. Empresa de Pesquisa Agropecuária - Embrapa Clima Temperado, Br 392, Km 78, Caixa Postal 403, Pelotas, 96010-971, Brazil

    Ângela Diniz Campos

  4. Departamento de Físico-Química, Instituto de Química, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Porto Alegre, RS, 91501-970, Brazil

    Irene Teresinha Santos Garcia

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  1. Fabiane Grecco da Silva Porto
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da Silva Porto, F.G., dos Santos Vasconcelos, E., Campos, Â.D. et al. Chitosan in Eucalyptus grandis Pyroligneous Liquor for Agricultural Application: Physicochemical and Structural Characterization During Storage. J Polym Environ 29, 1591–1599 (2021). https://doi.org/10.1007/s10924-020-01980-9

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