Functionalization of Extracellular Polymers of Pseudomonas aeruginosa N6P6 for Synthesis of CdS Nanoparticles and Cadmium Bioadsorption
Functionalization of the extracellular polymers (EPS) of a marine bacterium Pseudomonas aeruginosa N6P6 was carried out for cadmium (Cd) bioadsorption from an aqueous solution which led to the synthesis of cadmium sulfide (CdS) nanoparticles (NPs). Characterization of pristine, functionalized and Cd-treated functionalized EPS was accomplished by ATR-FTIR spectroscopy which illustrated Cd binding with the sulfhydryl (–SH) group. The XRD pattern confirmed the presence of CdS NPs on the functionalized EPS with diffraction peaks at 2θ = 27.45° and 32.66° indexed to (111) and (220) planes of cubic phase CdS. Maximum Cd adsorption was observed by the functionalized EPS which removed 88.86 ± 0.65% of Cd at pH 6.6 in 48 h. However, pristine EPS and bacterial cell biomass removed 83.61 ± 0.50% and 29.75 ± 0.73% of Cd respectively from aqueous solution. The experimental data of Cd adsorption thermodynamics by functionalized EPS fitted in Langmuir isotherm model. CdS NPs synthesis by functionalized EPS was evident by UV–Vis spectrum with a characteristic peak at 462 nm and Transmission electron microscopy with an average diameter of 8–10 nm. This work delivers an environment-friendly approach for efficient Cd removal from aqueous solution in the form of CdS NPs synthesis.
KeywordsMarine bacteria Extracellular polymers Functionalization Cadmium CdS NPs Bioadsorption
Authors would like to acknowledge the authorities of NIT, Rourkela for providing facilities. S. D. thanks the Department of Biotechnology, Government of India for research grants on marine bacterial biofilm-based enhanced bioremediation of PAHs and heavy metals. We would like to acknowledge Bose Institute, Kolkata for providing the AAS facility.
- 2.Faroon O, Ashizawa A, Wright S, Tucker P, Jenkins K, Ingerman L, Rudisill C (2012) Toxicological Profile for Cadmium. In: U.S. Department of Health and Human Services, Public Health Service. Centers for Disease Control and Prevention, Agency for Toxic Substances and Disease Registry (ATSDR), Atlanta, GA, USA, pp 1–487Google Scholar
- 9.Guibaud G, Bhatia D, d’Abzac P, Bourven I, Bordas F (2012) Cd(II) and Pb(II) sorption by extracellular polymeric substances (EPS) extracted from anaerobic granular biofilms: evidence of a pH sorption-edge. J Taiwan Inst Chem Eng 43:444–449. https://doi.org/10.1016/j.jtice.2011.12.007 CrossRefGoogle Scholar
- 16.Matlochová A, Plachá D, Rapantová N (2013) The application of nanoscale materials in groundwater remediation. Pol J Environ Stud 22:1401–1410Google Scholar
- 21.Bian R, Joseph S, Cui L, Pan G, Li L, Liu X, Zhang A, Rutlidge H, Wong S, Chia C, Marjo C, Gong B, Munroe P, Donne C (2014) A three-year experiment confirms continuous immobilization of cadmium and lead in contaminated paddy field with biochar amendment. J Hazard Mater 272:121–128. https://doi.org/10.1016/j.jhazmat.2014.03.017 CrossRefPubMedGoogle Scholar
- 24.Mangwani N, Kumari S, Das S (2015) Involvement of quorum sensing genes in biofilm development and degradation of polycyclic aromatic hydrocarbons by a marine bacterium Pseudomonas aeruginosa N6P6. Appl Microbiol Biotechnol 99:10283–10297. https://doi.org/10.1007/s00253-015-6868-7 CrossRefPubMedGoogle Scholar
- 37.Schue M, Fekete A, Ortet P, Brutesco C, Heulin T, Schmitt-Kopplin P, Achouak W, Santaella C (2011) Modulation of metabolism and switching to biofilm prevail over exopolysaccharide production in the response of Rhizobium alamii to cadmium. PLoS ONE. https://doi.org/10.1371/journal.pone.0026771 CrossRefPubMedPubMedCentralGoogle Scholar
- 39.Pavia DL, Lampman GM, Kriz GS, Vyvyan JA (2000) Introduction to spectroscopy, 4th edn. Cengage Learning, WashingtonGoogle Scholar
- 40.Cullity BD, Stock SR (2001) Elements of X-ray diffraction. Addison-Wesley publishing company, Inc, MassachusettsGoogle Scholar
- 43.Kumar Meena A, Mishra GK, Kumar S, Rajagopal C, Nagar PN (2004) Adsorption of cadmium(II) Ions from aqueous solution using different adsorbents. J Sci Ind Res 63:410–416Google Scholar