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Optimization of Poly(dl-Lactic Acid) Degradation and Evaluation of Biological Re-polymerization

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Abstract

Poly(dl-lactic acid) or PLA is a biodegradable polymer. It has received much attention since it plays an important role in resolving the global warming problem. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported as having PLA depolymerase potential and being applicable to PLA biodegradation, which was used in this work. Therefore, this research demonstrates the important basic knowledge on the biological degradation process by the crude PLA-degrading enzyme from strain T16-1. Its re-polymerization was evaluated. The optimization of PLA degradation by statistical methods based on central composite design was determined. Approximately 6700 mg/l PLA powder was degraded by the crude enzyme under optimized conditions: an initial enzyme activity of 200 U/ml, incubated at 60 °C for 24 h released 6843 mg/l lactic acid with 82% conversion, which was similar to the commercial enzyme proteinase K (81%). The degradable products were re-polymerized repeatedly by using commercial lipase as a catalyst under a nitrogen atmosphere for 6 h. A PLA oligomer was achieved with a molecular weight of 378 Da (n = 5). This is the first report to demonstrate the high efficiency of the enzyme to degrade 100% of PLA powder and to show the biological recycling process of PLA, which is promising for the treatment and utilization of biodegradable plastic wastes in the future.

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Acknowledgements

This work received financial support from the matching fund between Thailand Research Fund through and Srinakharinwirot University (Grant No. TRG5680026) and under the Core-to-Core Program, which was financially supported by Japan Society for the Promotion of Science (JSPS), National Research Council of Thailand (NRCT), Vietnam Ministry of Science and Technology (MOST), the National University of Laos, Beuth University of Applied Sciences and Brawijaya University as well as, Thailand Toray science foundation.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Srinakharinwirot University, Watthana, Bangkok, 10110, Thailand

    Anupan Youngpreda, Titiporn Panyachanakul & Sukhumaporn Krajangsang

  2. Department of Microbiology, Faculty of Science, Kasetsart University, Jatujak, Bangkok, 10900, Thailand

    Vichien Kitpreechavanich

  3. Department of Biotechnology, Faculty of Agro-industry, Kasetsart University, Jatujak, Bangkok, 10900, Thailand

    Sarote Sirisansaneeyakul

  4. Department of Chemistry, Faculty of Science, Srinakharinwirot University, Watthana, Bangkok, 10110, Thailand

    Sunit Suksamrarn

  5. Department of Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan

    Shinji Tokuyama

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  1. Anupan Youngpreda
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  2. Titiporn Panyachanakul
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  3. Vichien Kitpreechavanich
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  4. Sarote Sirisansaneeyakul
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  5. Sunit Suksamrarn
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  6. Shinji Tokuyama
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  7. Sukhumaporn Krajangsang
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Corresponding author

Correspondence to Sukhumaporn Krajangsang.

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Youngpreda, A., Panyachanakul, T., Kitpreechavanich, V. et al. Optimization of Poly(dl-Lactic Acid) Degradation and Evaluation of Biological Re-polymerization. J Polym Environ 25, 1131–1139 (2017). https://doi.org/10.1007/s10924-016-0885-1

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  • DOI: https://doi.org/10.1007/s10924-016-0885-1

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