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Biodegradation of Natural Rubber and Natural Rubber Products by Streptomyces sp. Strain CFMR 7

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Abstract

The rubber degrading activity of Streptomyces sp. CFMR 7 whose whole genome sequence was recently determined was tested with non-vulcanized fresh latex and common vulcanized rubber products such as latex glove, latex condom and latex car tyre. The degradation activity was unequivocally demonstrated by scanning electron microscopy with respect to microbial colonization efficiency, disintegration of rubber material and biofilm formation after 3, 6 and 9 months of inoculation. Fourier transform infrared spectroscopy comprising the attenuated total reflectance analysis on these inoculated products revealed insights into the biodegradation mechanism of this strain whereby, a decrease in the number of cis -1,4 double bonds in the polyisoprene chain, the appearance of ketone and aldehyde groups formation indicating an oxidative attack at the double bond of rubber hydrocarbon. In the presence of strain Streptomyces sp. CFMR 7, gel permeation chromatography analysis revealed a significant shift of the molecular weight distribution to lower values. Clear decrease in the molecular weight was observed over 3, 6 and 9 months of cultivation on fresh latex samples compared to other vulcanized products. No shift in the molecular weight distribution was observed for non-inoculated control. These results clearly showed that Streptomyces sp. CFMR 7 was able to cleave the carbon backbone of poly (cis -1,4-isoprene). Although this strain was able to degrade both non-vulcanized and vulcanized rubber products, faster degradation was obtained with natural rubber and rubber products with low complexity.

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Acknowledgments

This study was supported by USM Research University Grant (1001/PBIOLOGI/815069). Nanthini J is grateful to MyBrain15 program by the Ministry of Education Malaysia (MOE) for providing fellowship.

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Correspondence to Kumar Sudesh.

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Nanthini, J., Sudesh, K. Biodegradation of Natural Rubber and Natural Rubber Products by Streptomyces sp. Strain CFMR 7. J Polym Environ 25, 606–616 (2017). https://doi.org/10.1007/s10924-016-0840-1

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Keywords

  • Streptomyces sp. CFMR 7
  • Rubber
  • Degradation
  • cis-1,4 double bonds