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Production of Poly(l-Lactide)-Degrading Enzyme by Amycolatopsis orientalis ssp. orientalis and Its Catalytic Ability in Biological Recycling of Poly(l-Lactide)

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Abstract

The fermentation conditions for poly(l-lactide) (PLA)-degrading enzyme production by Amycolatopsis orientalis ssp. orientalis were statistically optimized by response surface methodology. The optimal value of the most important factors was 0.39 % PLA and 0.34 % gelatin for 2.81 days of cultivation. Under these conditions, the model predicted a PLA-degrading activity of 155.30 U/l. The verification showed the production amount of 154.2 U/l. The crude enzyme from A. orientalis ssp. orientalis showed potent PLA-degrading ability, which is efficient for the biological recycling of PLA. Up to 4,000 mg/l of PLA granule was completely degraded within 5 days at 45 °C by the crude enzyme. l-lactic acid (600 mg/l) was obtained as a degradation product of PLA after only 2 h of incubation. The results indicated that the crude PLA-degrading enzyme from A. orientalis ssp. orientalis has the potential to degrade PLA to lactic acid for the recycling of PLA industry and waste disposal.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 31100046).

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Authors and Affiliations

  1. School of Life Science, Northeast Normal University, Changchun, 130024, China

    Fan Li, Yue Zhan, Dandan Liu, Dongbo Liu, Hongmei Xia & Shan Chen

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  1. Fan Li
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  2. Yue Zhan
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  3. Dandan Liu
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  4. Dongbo Liu
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  5. Hongmei Xia
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  6. Shan Chen
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Correspondence to Shan Chen.

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Li, F., Zhan, Y., Liu, D. et al. Production of Poly(l-Lactide)-Degrading Enzyme by Amycolatopsis orientalis ssp. orientalis and Its Catalytic Ability in Biological Recycling of Poly(l-Lactide). J Polym Environ 21, 1143–1149 (2013). https://doi.org/10.1007/s10924-013-0590-2

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  • DOI: https://doi.org/10.1007/s10924-013-0590-2

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