Journal of Polymers and the Environment

, Volume 18, Issue 3, pp 231–234 | Cite as

In Vitro Cytotoxicity of Amylose-Based Bioplastic for Packaging Applications

  • Sanchita Bandyopadhyay-Ghosh
  • Robert Jeng
  • Joydeep Mukherjee
  • Mohini Sain
Original paper


Amylose containing polysaccharides are one of the most abundant and inexpensive naturally occurring biopolymers. Therefore, they are one of the most promising candidates to produce substitute plastics, especially in packaging applications. To determine the suitability for packaging applications, cytotoxicity of a modified amylose based bioplastic was investigated using NIH 3T3 Fibroblast cells from observation of cell morphology and MTS assay. Chemical durability of the amylose based bioplastic film was also studied by ion release and pH measurement after immersing the film into water. In vitro cytotoxicity (Cell morphology study and MTS assay) showed that the amylose based bioplastic film has in vitro biocompatibility and can be used for packaging applications. The ion release and pH measurement also supported the results.


Amylose Biopolymer Bioplastics Biodegradable Biocompatibility Cytotoxicity Packaging 


  1. 1.
    Mali S, Victoria M, Grossmann E, Garcia MA, Martino MN, Zaritzky NE (2004) Food Hydrocoll 19:157–164CrossRefGoogle Scholar
  2. 2.
    Van Soest JJG, Vliegenthart JFG (1997) Trends Biotechnol 15(6):208–213CrossRefGoogle Scholar
  3. 3.
    Fama LA, Rojas M, Goyanes A, Gerschenson L (2005) LWT 38:631–639CrossRefGoogle Scholar
  4. 4.
    Lawton JW (1996) Carbohydr Polym 29:203–208CrossRefGoogle Scholar
  5. 5.
    Poutanen K, Forssell P (1996) TRIP 4–4:128–132Google Scholar
  6. 6.
    Laohakunjit N, Noomhorm A (2003) Starch 56:348–356CrossRefGoogle Scholar
  7. 7.
    Huang CB, Jeng R, Sain M, Saville B, Hubbes M (2006) Bioresources 1(2):257–269Google Scholar
  8. 8.
    Wallace KE, Hill RG, Pembroke JT, Brown CJ, Hatton PV (1999) J Mater Sci Mater Med 10:697–701CrossRefGoogle Scholar
  9. 9.
    Lin XY, Fan HS, Li XD, Tang M, Zhang XD (2005) Key Eng Mater 284–286:553–556CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Sanchita Bandyopadhyay-Ghosh
    • 1
  • Robert Jeng
    • 1
  • Joydeep Mukherjee
    • 2
  • Mohini Sain
    • 1
  1. 1.Centre for Biocomposites and Biomaterials ProcessingUniversity of TorontoTorontoCanada
  2. 2.The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick ChildrenUniversity of TorontoTorontoCanada

Personalised recommendations