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High-throughput Screening: Establishing Mathematical and Physical Models for Bio-target Immobilization

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In high-throughput screening, the immobilization of bio-target shows a significant effect on the target performance, especially the conformation. This, ultimately, has an influence on the screening quality. In present article, a series of mathematical and physical models for target immobilization were established. As shown, in the immobilization process, if the plot of C */Q versus C * is a straight-line, the drugs obtained from in vitro screening are probably applicable to in vivo environment. Otherwise, when the linearity exists between ln Q or Q and ln C *, particularly between Q and ln C *, additional competition-experiments of known ligands are necessary. In addition, the immobilization in multilayer form, as well as its impact on screening quality, was also discussed in this article (C *, the equilibrium concentration of bio-target; Q, the adsorbance).

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Authors and Affiliations

  1. Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, P.R. China

    Songjun Li, Wuke Li, Xiao Hao, Pingchu Chen & Guangfu Yang

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  1. Songjun Li
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  2. Wuke Li
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  3. Xiao Hao
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Correspondence to Songjun Li.

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Li, S., Li, W., Hao, X. et al. High-throughput Screening: Establishing Mathematical and Physical Models for Bio-target Immobilization. J Math Chem 41, 271–282 (2007). https://doi.org/10.1007/s10910-006-9073-1

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  • DOI: https://doi.org/10.1007/s10910-006-9073-1

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