Abstract
Using an extract from the leaves of the Moringa Oleifera tree as a sustainable reducing and stabilizing agent, this research details a unique method for synthesizing zinc oxide (ZnO) nanoparticles. With the synthesized ZnO nanoparticles, a sensitive electrochemical sensor for detecting dopamine was developed. By exploiting the bioactive components found in Moringa Oleifera leaf extract, green production of ZnO nanoparticles was achieved, providing a chemical-free alternative. The nanoparticles’ structural and morphological features were deduced with the aid of analytical methods like X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Ultra Violet Visible spectroscopy (UV–Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force spectroscopy (AFM) and X-ray photoelectron spectroscopy (XPS). The electrochemical sensor was built by incorporating the synthesized ZnO nanoparticles onto a Glassy Carbon Electrode. The sensor is sensitive and selective to dopamine, a neurotransmitter involved in many physiological and neurological functions. We employed electrochemical methods like cyclic voltammetry, square wave voltammetry and electrochemical impedance spectroscopy to analyze dopamine molecules' interactions with the ZnO nanoparticle-modified electrode. We found that neurotransmitter binding affects the sensor's electrical response. The vast linear detection range and low dopamine detection limit make the electrochemical sensor helpful for basic neuroscience research and clinical diagnosis. ZnO nanoparticles synthesized ecologically showed promise as efficient sensing materials and the practicality of such approaches. As a whole, this research demonstrates the complementary relationship between green chemistry, nanotechnology, and electrochemical sensing, with potential for use in the creation of environmentally friendly sensors and the enhancement of dopamine detection.
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References
M.M. Ahmed, R. Zhao, J. Du, J. Li, J. Electrochem. Soc. 169, 20573 (2022)
S. Tajik, H. Beitollahi, S.Z. Mohammadi, M. Azimzadeh, K. Zhang, Q. Van Le, Y. Yamauchi, H.W. Jang, M. Shokouhimehr, RSC Adv. 10, 30481 (2020)
H. Beitollahi, M. Safaei, S. Tajik, Anal. Bioanal. Chem. Res. 6, 81 (2019)
N. Atta, A. Galal, F. Abu-Attia, S. Azab, J. Electrochem. Soc. 157, F116 (2010)
R. Tandel, S. Pawar, S. Jaldappagari, J. Electrochem. Soc. 163, H705 (2016)
S. Yasmin, M.S. Ahmed, D. Park, S. Jeon, J. Electrochem. Soc. 163, B491 (2016)
G. Li, P. Zhong, Y. Ye, X. Wan, Z. Cai, S. Yang, Y. Xia, Q. Li, J. Liu, Q. He, J. Electrochem. Soc. 166, B1552 (2019)
S. Singh, M.R. Hasan, P. Sharma, J. Narang, Sens. Int. 3, 100190 (2022)
S. Tajik, H. Beitollahi, F.G. Nejad, M. Safaei, K. Zhang, Q. Van Le, R.S. Varma, H.W. Jang, M. Shokouhimehr, RSC Adv. 10, 21561 (2020)
N. Anzar, R. Hasan, M. Tyagi, N. Yadav, J. Narang, Sens. Int. 1, 100003 (2020)
F. Garkani Nejad, S. Tajik, H. Beitollahi, I. Sheikhshoaie, Talanta 228, 122075 (2021)
M. Gerard, A. Chaubey, B.D. Malhotra, Biosens. Bioelectron. 17, 345 (2002)
C.B.A. Hassine, H. Kahri, H. Barhoumi, J. Electrochem. Soc. 167, 27516 (2020)
L. Gao, J. Ma, J. Zheng, J. Electrochem. Soc. 167, 107503 (2020)
H.R. Ghorbani, F.P. Mehr, H. Pazoki, B.M. Rahmani, Orient. J. Chem. 31, 1219–1221 (2015)
P. Sharma, M.R. Hasan, N.K. Mehto, A.B. Deepak, J. Narang, Sens. Int. 3, 100182 (2022)
P. Sharma, H. Hassan, M.R. Hasan, T. Fatima, H. Mohan, M. Khanuja, S. Kaushik, J. Narang, Biosens. Bioelectron. X 13, 100303 (2023)
N.K. Mehto, P. Sharma, S. Kumar, M. Khanuja, R. Rawal, J. Narang, Process Biochem. 123, 36 (2022)
B.D. Malhotra, A. Chaubey, Sens. Actuators B Chem. 91, 117 (2003)
N. Anzar, M. Rahil Hasan, M. Akram, N. Yadav, J. Narang, Process Biochem. 94, 126 (2020)
N. Anzar, S. Suleman, H. Bano, S. Parvez, M. Khanuja, R. Pilloton, J. Narang, Sensors 23, 5519 (2023)
J.N. Hasnidawani, H.N. Azlina, H. Norita, N.N. Bonnia, S. Ratim, E.S. Ali, Procedia Chem. 19, 211 (2016)
P.P. Mahamuni, P.M. Patil, M.J. Dhanavade, M.V. Badiger, P.G. Shadija, A.C. Lokhande, R.A. Bohara, Biochem. Biophys. Rep. 17, 71 (2019)
S. Sharma, S.S. Pande, P. Swaminathan, RSC Adv. 7, 39411 (2017)
S. Srujana, D. Bhagat, Nanotechnol. Environ. Eng. 7, 269 (2022)
G. Ghoshal, M. Singh, Mater. Sci. Energy Technol. 5, 22 (2022)
J.O. Primo, C. Bittencourt, S. Acosta, A. Sierra-Castillo, J.F. Colomer, S. Jaerger, V.C. Teixeira, F.J. Anaissi, Front. Chem. 8, 1 (2020)
N. Matinise, X.G. Fuku, K. Kaviyarasu, N. Mayedwa, M. Maaza, Appl. Surf. Sci. 406, 339 (2017)
S. Faisal, H. Jan, S.A. Shah, S. Shah, A. Khan, M.T. Akbar, M. Rizwan, F. Jan, N. Wajidullah, A.K. Akhtar, S. Syed, ACS Omega 6, 9709 (2021)
S. Saeed, S. Nawaz, A. Nisar, T. Mehmood, M. Tayyab, M. Nawaz, S. Firyal, M. Bilal, A. Mohyuddin, A. Ullah, Mater. Res. Express 8, 35004 (2021)
M.S. Lakshmi Prabha Chandrasekar, B.D. Sethuraman, S. Mohandos, Int. J. Environ. Anal. Chem. 8, 1 (2023)
W.H. Zhou, H.H. Wang, W.T. Li, X.C. Guo, D.X. Kou, Z.J. Zhou, Y.N. Meng, Q.W. Tian, S.X. Wu, J. Electrochem. Soc. 165, G3001 (2018)
F. Li, B. Ni, Y. Zheng, Y. Huang, G. Li, Surf. Interfaces 26, 101375 (2021)
J. Lin, B. Huang, Y. Dai, J. Wei, Y. Chen, Mater. Sci. Eng. C 93, 739 (2018)
S. Verma, P. Arya, A. Singh, J. Kaswan, A. Shukla, H.R. Kushwaha, S. Gupta, S.P. Singh, Biosens. Bioelectron. 165, 112347 (2020)
C. Nong, B. Yang, X. Li, S. Feng, H. Cui, Int. J. Electrochem. Sci. 17, 220331 (2022)
J.K. Shashikumara, B.E.K. Swamy, S.C. Sharma, S.A. Hariprasad, K. Mohanty, Sci. Rep. 11, 14310 (2021)
C. Yang, C. Zhang, T. Huang, X. Dong, L. Hua, J. Mater. Sci. 54, 14897 (2019)
S. Tajik, H. Beitollahi, H.W. Jang, M. Shokouhimehr, Talanta 232, 122379 (2021)
S. Tajik, Y. Orooji, F. Karimi, Z. Ghazanfari, H. Beitollahi, M. Shokouhimehr, R.S. Varma, H.W. Jang, J. Food Meas. Charact. 15, 4617 (2021)
S. Tajik, M.A. Taher, H. Beitollahi, Ionics 20, 1155 (2014)
M.M. Foroughi, H. Beitollahi, S. Tajik, A. Akbari, R. Hosseinzadeh, Int. J. Electrochem. Sci. 9, 8407 (2014)
A. Munawar, F. Zafar, S. Majeed, M. Irfan, H. Ullah Khan, G. Yasmin, N. Akhtar, J. Electroanal. Chem. 895, 115469 (2021)
G. Qu, G. Fan, M. Zhou, X. Rong, T. Li, R. Zhang, J. Sun, D. Chen, ACS Omega 4, 4221 (2019)
A. Sahai, N. Goswami, AIP Conf. Proc. 1665, 0500 (2015)
Z. Li, H. Chen, W. Liu, Catalysts 8, 557 (2018)
K. Elumalai, S. Velmurugan, Appl. Surf. Sci. 345, 329 (2015)
S.A. Al-Zahrani, M.B. Patil, S.N. Mathad, A.Y. Patil, A.A. Otaibi, N. Masood, D. Mansour, A. Khan, A. Manikandan, E. Syafri, Crystals 13, 577 (2023)
S.S. Kanmani, K. Ramachandran, S. Umapathy, Int. J. Photoenergy 2012, 1 (2012)
D. Letsholathebe, F.T. Thema, K. Mphale, K. Maabong, C.M. Magdalane, Mater. Today Proc. 38, 2445 (2020)
M. Manikandan, C. Revathi, P. Senthilkumar, S. Amreetha, S. Dhanuskodi, R.T. Rajendra Kumar, Ionics 26, 2003 (2020)
D. Balram, K.Y. Lian, N. Sebastian, Int. J. Electrochem. Sci. 13, 1542 (2018)
M. Manikandan, S. Dhanuskodi, N. Maheswari, G. Muralidharan, C. Revathi, R.T. Rajendra Kumar, G. Mohan Rao, Sens. Bio-Sens. Res. 13, 40 (2017)
M. Ognjanović, D.M. Stanković, M. Jović, M.P. Krstić, A. Lesch, H.H. Girault, B. Antić, A.C.S. Appl, Nano Mater. 3, 4654 (2020)
S. Palanisamy, S. Cheemalapati, S.M. Chen, Anal. Biochem. 429, 108 (2012)
W. Ma, D. Tian, Bioelectrochemistry 78, 106 (2010)
H.Y. Yue, H.J. Zhang, S. Huang, X.X. Lu, X. Gao, S.S. Song, Z. Wang, W.Q. Wang, E.H. Guan, Mater. Sci. Eng. C 108, 110490 (2020)
S.S.J. Aravind, S. Ramaprabhu, Nanosci. Methods 1, 102 (2012)
Acknowledgements
Authors are much thankful to Vellore Institute of Technology, Chennai for providing “VIT RGEMS SEED GRANT” for carrying out this research work.
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Authors are much thankful to Vellore Institute of Technology, Chennai for providing “VIT RGEMS SEED GRANT” for carrying out this research work.
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Author 1 (MM): Material synthesis, data collection, TEM, FESEM and electrochemical analysis. Author 2 (GLP): Conceived the proposed idea, contributed to the study conception, methods, and material selection. Author 3 (EM): XRD, FT-IR, UV–Vis characterization studies. Author 4 (VS): XPS, AFM analysis. All the authors have contributed to the overall paper drafting, extraction of results and editing work for their respective sections. All authors reviewed the manuscript.
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Manikandan, M., Priya, G.L., Manikandan, E. et al. ZnO Nanoparticle-Enhanced Electrochemical Sensor Utilizing Moringa Oleifera Leaf Extract for Real-Time Dopamine Detection. J Inorg Organomet Polym (2024). https://doi.org/10.1007/s10904-023-02955-8
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DOI: https://doi.org/10.1007/s10904-023-02955-8