Abstract
Synthesis of nanoparticle (NP) using biological systems is drawing attention of various research groups. Although, various biological agents can be used for synthesis of NP, however, plant extract mediated synthesis is gaining attention due to eco-friendly in nature and also cost-effective. The present work intends to synthesize silver nanoparticle (AgNP) using the fleshy pericarp of pomegranate (Punica granatum L.) which is rich in polyphenols and flavonoids. Initially, the biosynthesis of AgNP was confirmed observing the absorption peak at 462 nm using UV–visible spectroscope. The XRD data revealed that, the synthesized AgNP has face-centred cubic structure. Additionally, FE-SEM analysis demonstrated that biosynthesized AgNP was found to be spherical in shape with size in the range of 40–80 nm. The antioxidant activity was evaluated using DPPH assay, which demonstrated that AgNP is a good antioxidant agent. The antimicrobial activity of AgNP was further evaluated against Gram-positive and Gram-negative bacteria using growth kinetics and CFU count techniques and found that the antimicrobial activity of AgNP increases with increasing the concentration of AgNP. Additionally, the anti-biofilm activity of AgNP was evaluated against biofilm producing bacteria Staphylococcus aureus, which confirmed that AgNP has strong anti-biofilm activity against biofilm forming bacteria.
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Sahoo, B., Panigrahi, L.L., Das, R.P. et al. Biogenic Synthesis of Silver Nanoparticle from Punica granatum L. and Evaluation of Its Antioxidant, Antimicrobial and Anti-biofilm Activity. J Inorg Organomet Polym 32, 4250–4259 (2022). https://doi.org/10.1007/s10904-022-02441-7
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DOI: https://doi.org/10.1007/s10904-022-02441-7