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Biological Compound Capping of Silver Nanoparticle with the Seed Extracts of Blackcumin (Nigella sativa): A Potential Antibacterial, Antidiabetic, Anti-inflammatory, and Antioxidant

Journal of Inorganic and Organometallic Polymers and Materials Aims and scope Submit manuscript

Abstract

It is a well explored facts that need of biosynthesized nanomaterials has emerged for its several advantages in biomedical applications and the search for potential biosynthesized-nanomaterials is still ongoing. In this study an attempt was made to meet the requirement of potential biosynthesized-silver nanoparticles using the aqueous seed extract of N. sativa (Bc-AgNPs). X-ray powder diffraction (XRD) confirms the biosynthesis of silver nanoparticle (supports the observation of UV–Vis spectroscopy) with face centred cubic structure. Fourier-transform infrared spectroscopy (FTIR) confirms the capping of the active ingredients of seed extract and further the average size of 34 nm was confirmed by high-resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray analysis (EDX) confirms the purity of Bc-AgNPs. Due to the capping of the active ingredients of seed extract, the Bc-AgNPs have shown significant antibacterial activity against Gram negative bacteria and at the lower concentration of 100 µg*ml−1 it has 89% scavenging activity against free radicals. A strong anti-inflammatory (membrane stabilization) activity towards RBCs (98% at 100 µg*ml−1) was recorded when compared to the positive control (aceclofenac). In addition to the previous activities, a greater inhibition of carbohydrate hydrolyzing enzymes such as α-amylase, α-glucosidase and DPP-IV highlighted the antidiabetic potential of these green synthesized AgNPs. Overall, the efficient bioactivities of Bc-AgNPs advocates its future potential in biomedical products.

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Abbreviations

AgNO3 :

Silver nitrate

ATCC:

American Type Culture Collection

BC:

Black cumin

Bc-AgNPs:

Black cumin seed extracts capped silver nanoparticles

CLSI:

Clinical and Laboratory Standards Institute

DM:

Diabetes mellitus

DNS:

DiNitroSalicylic acid

DPPH-2:

Diphenyl-1-picrylhydrazyl

DPP-IV:

Dipeptidyl peptidase IV

EDX:

Energy dispersive X-ray analysis

FTIR:

Fourier-transform infrared spectroscopy

GC–MS:

Gas chromatography-Mass spectrophotometry

HR-TEM:

High-resolution transmission electron microscopy

HRBC:

Human red blood cells

LB:

Luria–Bertani (LB) agar

MBC:

Minimum bactericidal concentration

MIC:

Minimum inhibitory concentration

MNP:

Metal nanoparticles

N. sativa :

Nigella sativa

NSE:

Nigella sativa Seed extracts

SPR:

Surface plasmon resonance

UV:

UV–Vis spectroscopy

XRD:

X-ray powder diffraction

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Acknowledgements

The corresponding author Dr. Sekar Vijayakumar thank to Shandong University for providing the opportunity for post doctoral fellowship. Prof.Esteban F. Durán-Lara thanks the FONDECYT Projects Number 11170155.

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Authors and Affiliations

  1. Marine College, Shandong University, Weihai, 264209, People’s Republic of China

    Sekar Vijayakumar & Jingdi Chen

  2. Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science campus 6th Floor, Karaikudi, Tamil Nadu, 630004, India

    Sekar Vijayakumar, Mani Divya & Baskaralingam Vaseeharan

  3. Vermiculture Technology Lab, Department of Animal Health and Management, Alagappa University, Science campus 6th Floor, Karaikudi, Tamil Nadu, 630004, India

    Muniyandi Biruntha

  4. Embrapa Genetic Resources and Biotechnology, PBI, Laboratory of Nanobiotechnology (LNANO), Brasília, DF, 70770-917, Brazil

    Luciano Paulino Silva

  5. Bio & NanoMaterials Lab| Drug Delivery and Controlled Release, Universidad de Talca, 3460000, Talca, Maule, Chile

    Esteban F. Durán-Lara

  6. Departamento de Microbiología, Facultad de Ciencias de la Salud, Universidad de Talca, 3460000, Talca, Maule, Chile

    Esteban F. Durán-Lara

  7. Department of Physics, Sri Sarada College for Women (Autonomous), Salem, Tamil Nadu, 636016, India

    Krishnan Shreema

  8. Institute for Intelligent Systems, Faculty of Engineering and the Built Environment, University of Johannesburg, Johannesburg, South Africa

    Shivendu Ranjan & Nandita Dasgupta

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  1. Sekar Vijayakumar
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  2. Mani Divya
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  3. Baskaralingam Vaseeharan
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Correspondence to Sekar Vijayakumar.

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Vijayakumar, S., Divya, M., Vaseeharan, B. et al. Biological Compound Capping of Silver Nanoparticle with the Seed Extracts of Blackcumin (Nigella sativa): A Potential Antibacterial, Antidiabetic, Anti-inflammatory, and Antioxidant. J Inorg Organomet Polym 31, 624–635 (2021). https://doi.org/10.1007/s10904-020-01713-4

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