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A Novel Approach of Synthesis Zinc Oxide Nanoparticles by Bergenia ciliata Rhizome Extract: Antibacterial and Anticancer Potential

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Abstract

The synthesis of metal and semiconductor nanoparticles is an expanding research area due to the potential applications in the development of novel technologies. In this work reports novel biosynthesis of zinc oxide NPs by rhizome extract of plant B. ciliata. The biosynthesized zinc oxide NPs were characterized various techniques using UV–Vis, FTIR, XRD, DLS, SEM and EDX. UV–Visible absorbance spectrum of ZnO NPs exhibited the absorbance band at 340 nm. The XRD data showed the crystalline nature of the nanoparticles. DLS shows average particle size was around 30 nm. Morphology observed by SEM analysis confirming the flower shaped nanoparticle and EDX spectrum presence of Zn and O in the synthesized NPs. The synthesized ZnO NPs showed antimicrobial effects against Gram negative microorganisms with lowest MIC value recorded as 6.25 μg/mL. Free radical scavenging capacity of zinc oxide nanoparticles were valued using ABTS and DPPH assay with changing the nanoparticles concentration, which showed scavenging activity with IC50 = 63.8 and 118.7 µg/ mL−1 respectively. ZnO NPs showed a remarkable selective cytotoxicity against the Human Cervical cancer (HeLa) and Human colon cancer (HT-29) cell lines. The synthesis of ZnO nanoparticles from B. ciliata rhizome extracts by green synthesis and determination of the bioactivity of these nanoparticles were reported for the first time in this work.

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Acknowledgements

The authors acknowledge Vice-Chancellor, Shoolini University, Solan, for providing infrastructure support to conduct the research work. Authors are highly thankful to the School of Bioengineering and Food Technology, Shoolini University, Solan, India.

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Authors and Affiliations

  1. Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, Himachal Pradesh, 173229, India

    Kanika Dulta, Parveen Chauhan & P. K. Chauhan

  2. Department of Biology, Hacettepe University, Ankara, Turkey

    Gözde Koşarsoy Ağçeli

  3. Faculty of Physics and Material Science, Shoolini University, Solan, Himachal Pradesh, 173229, India

    Rohit Jasrotia

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  1. Kanika Dulta
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Dulta, K., Koşarsoy Ağçeli, G., Chauhan, P. et al. A Novel Approach of Synthesis Zinc Oxide Nanoparticles by Bergenia ciliata Rhizome Extract: Antibacterial and Anticancer Potential. J Inorg Organomet Polym 31, 180–190 (2021). https://doi.org/10.1007/s10904-020-01684-6

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