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Nano-Sized MIL-100(Fe) as a Carrier Material for Nitidine Chloride Reduces Toxicity and Enhances Anticancer Effects In Vitro

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Abstract

Nitidine chloride (NC) is a natural alkaloid and has strong antitumor activity. However, its clinical application is limited due to the observed non-specific toxicity and low bioavailability. In this study, we synthesized nanoscale metal–organic frameworks MIL-100(Fe), which was used as a nanocarrier to deliver NC. MIL-100(Fe) was characterized by X-ray diffraction (XRD), Fourier transform infrared spectrometer (FT-IR), Brunauer–Emmett–Teller (BET), dynamic light scattering (DLS), and scanning electron microscopy (SEM). NC was encapsulated in MIL-100(Fe) with a high loading capacity of 33.43 wt%. It shown that NC has progressive releasing behavior with 68% in 4 days under phosphate-buffered saline. The in vitro cytotoxicity of free NC and NC@MIL-100(Fe) were investigated by the MTT assay using the healthy liver cell line (LO2) and a liver cancer cell line (HepG2). Interestingly, NC@MIL-100(Fe) exhibited low toxicity on LO2 cells and high cytotoxicity in HepG2 cells compared to free NC.

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Acknowledgements

This work is supported by Guangxi Natural Science Foundation (No. 2016GXNSFAA380063) and Guangxi Science Foundation (No. AD18126005) and and Special Funds for Scientific Technological Innovation of Undergraduates in Guangdong Province (pdjh2019b0222, pdjh2019b0219, pdjh2019b0221 and pdjh2020b0267).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning, Guangxi, 530006, China

    JiaRui Bi, Yu Zheng & Jian Wu

  2. Guangdong Key Laboratory for Research and Development of Natural Drugs, Key Laboratory of Research and Development of New Medical Materials of Guangdong Medical University, School of Pharmacy, Guangdong Medical University, Dongguan, 523808, China

    LiQing Fang, YuCheng Guan & AiQing Ma

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  1. JiaRui Bi
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  2. Yu Zheng
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Correspondence to Jian Wu.

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Bi, J., Zheng, Y., Fang, L. et al. Nano-Sized MIL-100(Fe) as a Carrier Material for Nitidine Chloride Reduces Toxicity and Enhances Anticancer Effects In Vitro. J Inorg Organomet Polym 30, 3388–3395 (2020). https://doi.org/10.1007/s10904-020-01548-z

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