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A Novel Ship-in-Bottle Type Immobilized HRP via Co-adsorption of Super Paramagnets and HRP into Silica Hollow Fiber

  • Na Li
  • Hongxia Yin
  • Jicong Pei
  • Yan Huang
  • Guangtong Xu
  • Hongmin Yuan
  • Xiangnong Liu
  • Yuxiang Yang
Article
  • 87 Downloads

Abstract

In this paper, two type silica hollow fibers were synthesized through a soft template and hard template directing route respectively, and the super paramagnets was synthesized by thermal decomposition. The obtained silica hollow fiber and super paramagnets were characterized by XRD, N2 adsorption, SEM, TEM, EDS and XPS respectively. The ship-in-bottle type immobilized horseradish peroxidase (HRP) was assembled by co-adsorption of super paramagnets and HRP into the pore channel of silica hollow fiber in reverse microemulsion media. The effects of various parameters on enzyme activity of ship-in-bottle type immobilized HRP, including morphology, water content (ω0), super paramagnets concentration, HRP concentration, adsorption time, pH, crosslinking time and glutaraldehyde concentration were discussed in detailed. Moreover, the degradation performance of dichlorodiphenyltrichloroethane (DDT) by HRP immobilized on silica hollow fiber and spherical nano-silica was evaluated.

Graphical Abstract

The ship-in-bottle type immobilized HRP have been prepared by uniformly co-adsorption of super paramagnets and HRP into the pore channel of silica hollow fiber with high enzyme activity, as evidenced by HRTEM characterization, which can be employed to degrade DDT to low toxic organic compound with low molecular weight.

Keywords

Silica hollow fiber Super paramagnets Immobilized horseradish peroxidase Ship-in-bottle 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (20971043), State Key Laboratory of Catalytic Materials and Reaction Engineering (RIPP, SINOPEC), the Open Project Program of State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, and the Fundamental Research Funds for the Central Universities.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.School of Chemistry and Molecular EngineeringEast China University of Science and TechologyShanghaiChina
  2. 2.State Key Laboratory of Catalytic Materials and Reaction Engineering (RIPP, SINOPEC)BeijingChina
  3. 3.State Key Laboratory of Inorganic Synthesis and Preparative ChemistryJilin UniversityChangchunChina
  4. 4.Analysis Test CenterYangzhou UniversityYangzhouChina

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