d-Maltose Synthesized Silver Nanoparticles for Biofilm Eradication



Biofilms are complex bacterial communities have a mechanism for antibiotic resistance leading to human health problems. It remains challenging to treat and eradicate biofilms. In this work, the use of d-maltose synthesized silver nanoparticles (AgNPs) was investigated in an effort to eradicate a biofilm. AgNPs were synthesized using a modified Tollen’s method. d-maltose was used in synthesizing AgNPs with different concentrations of d-maltose (0.01, 0.05 and 0.1 M), referred to as NP1, NP2 and NP3, respectively. TEM images revealed that the particles were polygon shaped. The particle sizes were found to be 86.81 ± 13.39, 54.94 ± 11.63 and 31.43 ± 31.76 nm depending on their sugar concentrations. UV–Vis, ATR–FTIR, and XRD patterns were employed to characterize the AgNPs. Then, these AgNPs were investigated for their anti-bacterial effects against Escherichia coli and Staphylococcus aureus. Evaluation of the minimum inhibitory concentration and minimal bactericidal concentration revealed that S. aureus was inhibited by all AgNPs and killed by NP1 and NP3, and E. coli was inhibited and killed at all AgNPs doses. Furthermore, anti-biofilm activity against these two bacteria was observed using SEM and confocal laser scanning microscopy. This sugar coated AgNPs is a promising material for use in eradication of biofilms.


Silver nanoparticles Green synthesis Anti-biofilm Antibacterial activity 



The authors would like to acknowledge the financial support by the Thailand Research Fund (MRG5980137) and the Nanotechnology Center (NANOTEC), NSTDA, Ministry of Science and Technology, Thailand, through its program of Center of Excellence Network.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Materials Science and Nanotechnology Program, Faculty of ScienceKhon Kaen UniversityKhon KaenThailand
  2. 2.Biomedical Sciences, Graduate SchoolKhon Kaen UniversityKhon KaenThailand
  3. 3.Division of Clinical Microbiology, Faculty of Associated Medical SciencesKhon Kaen UniversityKhon KaenThailand
  4. 4.The Center for Research & Development of Medical Diagnostic Laboratories, Faculty of Associated Medical SciencesKhon Kaen UniversityKhon KaenThailand
  5. 5.Nanotec-KKU Center of Excellence on Advanced Nanomaterials for Energy Production and StorageKhon KaenThailand
  6. 6.Department of Chemistry, Faculty of ScienceRamkhamhaeng UniversityBangkokThailand

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