Skip to main content
SpringerLink
Log in

Synthesis of Core/Shell Magnetic Porous Microspheres for Lipase Immobilization

  • Published:
Journal of Inorganic and Organometallic Polymers and Materials Aims and scope Submit manuscript

Abstract

Magnetic porous hydrophobic microspheres were prepared by modified suspension copolymerization of methacrylate (MMA) and divinylbenzene (DVB) in the presence of oleic acid coated magnetite (Fe3O4), and the microspheres were used as biocarrier for the lipase immobilization. The results showed that the magnetic microspheres possessed spherical shape, core/shell structure, porous structure and high magnetic content, and the size and structure of magnetic microspheres had no significant changes after enzyme binding. The particle average size of microspheres was 66 μm, the magnetic content of microspheres was up to 31%, and the magnetization saturation values of the core/shell magnetic microspheres were measured at 300 K to be 11.02 emu g−1. Lipase was immobilized on the magnetic porous carrier at up to 16.30 mg/g carrier. Activity and enantioselectivity of the immobilized lipase for the synthesis of R-HMPC acetate were investigated, indicating an interfacial activation of the enzyme after immobilization. Moreover, the pH dependency and operational stability of the immobilized lipase were studied, and they possess high stability and can be reused for ten cycles with loss 10% activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  1. E. Anderson, K. Larsson, O. Kirk, Biocatal. Biotransform. 16, 181 (1998)

    Article  CAS  Google Scholar 

  2. R.B.V. Gotor-Fernández, V. Gotor, J. Mol. Catal. B: Enzyme 40, 111 (2006)

    Article  Google Scholar 

  3. M. Ellrichmann, M. Kapelle, P. Ritter, J. Holst, K. Herzig, W. Schmidt, F. Schmitz, J. Meier, J. Clin. Endocrinol. Metab. 93, 3995 (2008)

    Article  CAS  Google Scholar 

  4. Y. Zhang, N. Kohler, M. Zhang, Biomaterials 23, 1553 (2002)

    Article  CAS  Google Scholar 

  5. A. Gupta, M. Gupta, Biomaterials 26, 3995 (2005)

    Article  CAS  Google Scholar 

  6. Y. Deng, W. Yang, C. Wang, S. Fu, Adv. Mater. 15, 1729 (2003)

    Article  CAS  Google Scholar 

  7. R. Arshady, Biomaterials 14, 5 (1993)

    Article  CAS  Google Scholar 

  8. S. Akg l, Y. Kacar, A. Denizli, M. Arica, Food Chem. 74, 281 (2001)

    Article  Google Scholar 

  9. Q. Liu, L. Wang, A. Xiao, J. Gao, W. Ding, H. Yu, J. Huo, M. Ericson, J. Hazard. Mater. 181, 586 (2010)

    Article  CAS  Google Scholar 

  10. C.T. Yavuz, J. Mayo, W.W. Yu, A. Prakash, J.C. Falkner, S. Yean, L. Cong, H.J. Shipley, A. Kan, M. Tomson, Science 314, 964 (2006)

    Article  Google Scholar 

  11. F.Y. Cheng, C.H. Su, Y.S. Yang, C.S. Yeh, C.Y. Tsai, C.L. Wu, M.T. Wu, D.B. Shieh, Biomaterials 26, 729 (2005)

    Article  CAS  Google Scholar 

  12. Z. Guo, Y. Sun, Biotechnol. Prog. 20, 500 (2004)

    Article  CAS  Google Scholar 

  13. D. Bozhinova, B. Galunsky, G. Yueping, M. Franzreb, R.K. ster, V. Kasche, Biotechnol. Lett 26, 343 (2004)

    CAS  Google Scholar 

  14. S.A.N. Öztürk, M. ArIsoy, A. Denizli, Sep. Purif. Technol. 58, 83 (2007)

    Article  Google Scholar 

  15. G. Bayramoglu, B. Kaya, M. Yakup ArIca, Food Chem. 92, 261 (2005)

    Article  CAS  Google Scholar 

  16. Z. Guo, S. Bai, Y. Sun, Enzyme Microb. Technol. 32, 776 (2003)

    CAS  Google Scholar 

  17. A.S.J. Krízová, B. Rittich, D. Horák, J. Chromatogr. A 1064, 247 (2005)

    Article  Google Scholar 

  18. S. Berensmeier, Appl. Microbiol. Biotechnol. 73, 495 (2006)

    Article  CAS  Google Scholar 

  19. J. Kandzia, M. Anderson, W. Müller-Ruchholtz, J. Cancer Res. Clin. Oncol. 101, 165 (1981)

    Article  CAS  Google Scholar 

  20. M. Kuhara, H. Takeyama, T. Tanaka, T. Matsunaga, Anal. Chem. 76, 6207 (2004)

    Article  CAS  Google Scholar 

  21. J. Chatterjee, Y. Haik, C. Chen, J. Magn. Magn. Mater. 225, 21 (2001)

    Article  CAS  Google Scholar 

  22. C. Xu, K. Xu, H. Gu, X. Zhong, Z. Guo, R. Zheng, X. Zhang, B. Xu, J. Am. Chem. Soc 126, 3392 (2004)

    Article  CAS  Google Scholar 

  23. J. Gass, P. Poddar, J. Almand, S. Srinath, H. Srikanth, Adv. Funct. Mater. 16, 71 (2006)

    Article  CAS  Google Scholar 

  24. S. Mitsuda, S. Nabeshima, H. Hirohara, Appl. Microbiol. Biotechnol. 31, 4 (1989)

    Article  Google Scholar 

  25. S. Mitsuda, S. Nabeshima, H. Hirohara, Appl. Microbiol. Biotechnol. 29, 4 (1988)

    Article  Google Scholar 

  26. G. Yang, J. Wu, G. Xu, L. Yang, J. Mol. Catal. B: Enzyme 57, 96 (2009)

    Article  CAS  Google Scholar 

  27. Z. Yang, D. Zacherl, A.J. Russell, J. Am. Chem. Soc. 115, 12251 (1993)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by the International Science and Technology Cooperation Project of Ministry of Science and Technology of China (2009DFR40640), Doctoral Foundation of Ministry of Education of China (200803350118), Science and Technology Program of Ningbo (2009D10005), Nature Science Foundation of Zhejiang Province (Z4100291), the National Natural Science Foundation of China (Key Program) (Grant No. 20936002), the National High Technology Research and Development Program of China (863 Program, 2010AA101502), National Basic Research Program of China (973 Program, 2011CB710800). We gratefully thank Prof. Neil R. Thomas at the University of Nottingham for helpful discussion.

Author information

Authors and Affiliations

  1. State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, People’s Republic of China

    Yulei Tai, Li Wang, Jingmin Gao, Weihong Tao, Jia Huo & Lirong Yang

  2. Oujiang College, Wenzhou University, Wenzhou, 325027, People’s Republic of China

    Yulei Tai

Authors
  1. Yulei Tai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jingmin Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Weihong Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jia Huo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lirong Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Li Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tai, Y., Wang, L., Gao, J. et al. Synthesis of Core/Shell Magnetic Porous Microspheres for Lipase Immobilization. J Inorg Organomet Polym 22, 213–222 (2012). https://doi.org/10.1007/s10904-011-9561-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10904-011-9561-z

Keywords

Search

Navigation