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A Novel Nanocomposite Matrix Based on Silylated Chitosan and Multiwall Carban Nanotubes for the Immobilization of Urease

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Abstract

A nanocomposite matrix made up of silylated chitosan and multiwall carbon nanotubes (CHIT–SiO2–MWCNTs) was fabricated to investigate the immobilization of urease (Urs). Urs enzyme was covalently immobilized with the CHIT–SiO2–MWCNTs matrix using glutaraldehyde as a linker. The resulting Urs/CHIT–SiO2–MWCNTs biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV) taking CHIT–SiO2–MWNTs as a reference. The influence of various parameters on Urs enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis–Menten constant and apparent activities for the Urs enzyme were calculated to be 0.51 mM and 89.02 mg/cm2, respectively; indicating CHIT–SiO2–MWCNTs nanocomposite matrix has a high affinity to immobilize Urs enzyme.

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Acknowledgments

Author is thankful to the Department of Science and Technology, Govt. of India for generous financial support and to the Director, National Physical Laboratory, New Delhi, India for providing infrastructure facilities to carry out this work.

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Authors and Affiliations

  1. Division of Engineering Materials, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi, 110 012, India

    Ashutosh Tiwari

  2. Department of Mechanical Engineering, University of Wisconsin-Milwaukee, 3200 North Cramer Street, Milwaukee, WI, 53211, USA

    Ashutosh Tiwari

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  1. Ashutosh Tiwari
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Correspondence to Ashutosh Tiwari.

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Tiwari, A. A Novel Nanocomposite Matrix Based on Silylated Chitosan and Multiwall Carban Nanotubes for the Immobilization of Urease. J Inorg Organomet Polym 19, 361–366 (2009). https://doi.org/10.1007/s10904-009-9280-x

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  • DOI: https://doi.org/10.1007/s10904-009-9280-x

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