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A Calcium(II)-Based l-Arginine for ATP Binding and Hydrolysis

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Abstract

The supramolecular recognition of Ca(II) and N α-4-tosyl-l-arginine methyl ester hydrochloride (TAME) with ATP were investigated using 1H and 31P NMR spectra. In the Ca(II)–ATP–TAME ternary system, Ca2+ and TAME bind with ATP via the phosphate chain and adenine ring of ATP. The binding forces are mainly electrostatic and cation (Ca2+)–π and π–π stacking interaction. Furthermore, the hydrolysis of ATP catalyzed by Ca(II) and TAME was studied at pH 7.0 and 60 °C using 31P NMR spectra. Kinetics studies show that the ATP hydrolysis rate constant is 0.1035 h−1 in the Ca(II)–TAME–ATP ternary system, whereas the value is 8.5 × 10−3 h−1 under the same conditions without TAME and Ca2+. The Ca(II) ions and TAME accelerate the ATP hydrolysis process about 12-fold. The proposed mechanism of ATP hydrolysis catalyzed by Ca2+–TAME occurs through an addition–elimination reaction sequence. These results can help us get more useful information at the molecular level about the key amino acid residue(s) and metal ions that serve as cofactors in the ATPase effect on ATP hydrolysis/synthesis.

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Acknowledgment

The authors thank for Ms. Xiaoning Wei for technical assistance in obtaining NMR spectra. This work was supported by the National Natural Science Foundation of China (No. 90210027).

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Authors and Affiliations

  1. State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China

    Yanqing Ma & Gongxuan Lu

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Yanqing Ma

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  1. Yanqing Ma
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  2. Gongxuan Lu
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Correspondence to Gongxuan Lu.

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Ma, Y., Lu, G. A Calcium(II)-Based l-Arginine for ATP Binding and Hydrolysis. J Inorg Organomet Polym 18, 435–440 (2008). https://doi.org/10.1007/s10904-008-9225-9

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  • DOI: https://doi.org/10.1007/s10904-008-9225-9

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