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Vitamin B6 Cofactor Pyridoxal 5’-phosphate Conjugated Papain-Stabilized Fluorescent Gold Nanoclusters for Switch-on Detection of Zinc(II)

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Abstract

In this study, fluorescent gold nanoclusters (AuNCs) conjugated with pyridoxal-5-phosphate (PLP) were synthesized, characterized, and used for Zn2+ fluorescence turn-on sensing. PLP was conjugated over the surface of papain-stabilized fluorescent gold nanoclusters (pap-AuNCs; λex = 380 nm, λem = 670 nm) by forming imine linkage. Due to this modification, the red color emitting pap-AuNCs changed to orange color emitting nanoclusters PLP_pap-AuNCs. The nano-assembly PLP_pap-AuNCs detect Zn2+ selectively by showing a notable fluorescence enhancement at 477 nm. Zn2+ detection with PLP_pap-AuNCs was quick and easy, with an estimated detection limit of 0.14 µM. Further, paper strips and cotton buds coated with PLP_pap-AuNCs were developed for affordable on-site visual detection of Zn2+. Finally, the detection of Zn2+ in actual environmental water samples served as validation of the usefulness of PLP_pap-AuNCs.

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Data Availability

No datasets were generated or analysed during the current study.

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Authors and Affiliations

  1. Department of Chemistry, Sardar Vallabhbhai National Institute Technology, Surat, Gujarat, 395007, India

    Jayant Chaudhary, Aditi Tripathi & Suban K. Sahoo

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  1. Jayant Chaudhary
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  2. Aditi Tripathi
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  3. Suban K. Sahoo
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Contributions

All authors contributed to the study. The Investigation, Validation, Formal analysis, Data curation, Writing-original draft were performed by Jayant Chaudhary and Aditi Tripathi. The Conceptualization, Resources, Supervision, and Writing-review & editing were performed by Suban K Sahoo.

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Correspondence to Suban K. Sahoo.

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Chaudhary, J., Tripathi, A. & Sahoo, S.K. Vitamin B6 Cofactor Pyridoxal 5’-phosphate Conjugated Papain-Stabilized Fluorescent Gold Nanoclusters for Switch-on Detection of Zinc(II). J Fluoresc (2024). https://doi.org/10.1007/s10895-024-03849-9

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