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Photophysical Properties of Sinapic Acid and Ferulic Acid and Their Binding Mechanism with Caffeine

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Abstract

Sinapic acid (SA) and ferulic acid (FA) are bioactive compounds used in the food, pharmaceutical, and cosmetic industries due to their antioxidant properties. In this work, we studied the photophysical properties of SA and FA in different solvents and concentrations and their interactions with caffeine (CF), using ultraviolet–visible (UV–Vis), fluorescence spectroscopy and Fourier transform infrared (FTIR) spectroscopy. The findings show that the quantum yield, fluorescence lifetime, radiative decay rates, and non-radiative decay rates of SA and FA are influenced by the concentrations and solvent polarity. The interaction between SA and FA with CF was also studied using UV–Vis and fluorescence spectroscopy. The results indicate that the CF quenched the fluorescence intensity of SA and FA by static quenching due to the formation of a non-fluorescent complex. The van't Hoff equation suggests that the van der Waals forces and hydrogen bonds force were responsible for the interaction between SA and CF, as indicated by a negative change in enthalpy (\({\Delta {\text{H}}}^{{\text{o}}}\) < 0) and a negative change in entropy (\({\Delta {\text{S}}}^{{\text{o}}}\) < 0). On the other hand, the interaction between FA and CF was primarily controlled by electrostatic force, as indicated by a negative change in enthalpy (\({\Delta {\text{H}}}^{{\text{o}}}\) < 0) and a positive change in entropy (\({\Delta {\text{S}}}^{{\text{o}}}\) > 0). The negative change in Gibbs free energy (\({\Delta {\text{G}}}^{{\text{o}}}\)) indicates that both compounds underwent a spontaneous binding process.

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Acknowledgements

The authors greatly acknowledge Gurumurthi Thilanaya (Prof) for his contribution to the purchase of Ferulic acid and Sinapic acid samples from Sigma Aldrich (India) for this work.

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Authors and Affiliations

  1. Department of Applied Physics, School of Applied Sciences, Adama Science and Technology University, P.O. Box 1888, Adama, Ethiopia

    Umer Sherefedin, Abebe Belay, Alemu Kebede, Alemayehu Getahun Kumela & Semahegn Asemare

  2. Department of Applied Physics, School of Applied Natural and Computational Sciences, Jinka University, P.O. Box 165, Jinka, Ethiopia

    Kusse Gudishe & Alemayehu Getahun Kumela

  3. Department of Applied Physics, College of Natural and Computational Sciences, Mekdela Amba University, Tullu Awulia, P.O. Box 32, Amhara, Ethiopia

    Kusse Gudishe & Alemayehu Getahun Kumela

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  1. Umer Sherefedin
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Contributions

Umer Sherefedin: Conceptualization, analysis, writing; editing, writing original draft. Abebe Belay: Conceptualization, supervision, reviewing and editing. Kusse Gudishe: Conceptualization, supervision, reviewing and editing, Alemu Kebede: Supervision, reviewing and editing, Alemayehu Getahun Kumela: Reviewing and editing. Semahegn Asemare: Reviewing and editing.

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Sherefedin, U., Belay, A., Gudishe, K. et al. Photophysical Properties of Sinapic Acid and Ferulic Acid and Their Binding Mechanism with Caffeine. J Fluoresc (2024). https://doi.org/10.1007/s10895-024-03689-7

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