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Determination of Lead in Bee Products by Solid Surface Fluorescence Using Complexation and Coacervation at Room Temperature Processes. An Environmental Friendly Methodology

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Abstract

An accurate, economic and green methodology for Pb(II) monitoring in bee products is proposed. Complexed metal traces were preconcentrated on Nylon membranes using the coacervation phenomenon based on room temperature reaction between the cationic surfactant hexadecyltrimethylammonium bromide and the bile salt sodium cholate. The increase in solid surface fluorescence signal of dyes 8-hydroxyquinoleine and o-phenanthroline due to Pb(II) presence was used for the metal quantification. Experimental variables that influence on preconcentration step and fluorimetric sensitivity were optimized using uni-varied assays. Pb(II) concentration was determined on membranes by solid surface fluorescence at λem = 470 nm (λexc = 445 nm), using a solid sample holder. The calibration at optimal experimental conditions showed a LOD of 4.2 × 10–4 mg Kg−1 with a linear range of 1.28 × 10–3 mg Kg−1 to 8.73 mg Kg−1 and was successfully applied to Pb(II) quantification in different bee products produced in central west region of Argentina. The proposed methodology was applied to all samples after appropriate dilution. Accuracy methodology was evaluated by comparison of the obtained results with those found by ICP-MS, with percentage relative error under 8%. The precision was better than 0.0344 CV for Pb(II) determination.

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Data Availability

No datasets were generated or analysed during the current study.

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Acknowledgements

Authors gratefully thank to Instituto de Química San Luis - Consejo Nacional de Investigaciones Científicas y Tecnológicas (INQUISAL CONICET, Project 11220130100605CO) and Universidad Nacional de San Luis (Project PROICO 02-1120), Argentina, for the financial support.

Funding

This work was supported by Instituto de Química San Luis - Consejo Nacional de Investigaciones Científicas y Tecnológicas (INQUISAL CONICET, Project 11220130100605CO) and Universidad Nacional de San Luis (Project PROICO 02-1120).

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Authors and Affiliations

  1. Área de Química Analítica Facultad de Química, Bioquímica y Farmacia UNSL, San Luis Argentina Chacabuco y Pedernera (CP: D5700HHW), San Luis, Argentina

    Liliana Patricia Fernández

  2. INQUISAL-CONICET, Almirante Brown 907 (CP: D5700HHW), San Luis, Argentina

    Maria Carolina Talio, Mariano Acosta & Liliana Patricia Fernández

  3. Área de Química General e Inorgánica Facultad de Química, Bioquímica y Farmacia UNSL, San Luis Argentina, Chacabuco y Pedernera (CP: D5700HHW), San Luis, Argentina

    Maria Carolina Talio & Mariano Acosta

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  1. Maria Carolina Talio
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Contributions

Maria Carolina Talio and Mariano Acosta and carried out the laboratory experiences, the calculations and the tables. Maria Carolina Talio y Liliana Patricia Fernández wrote the main manuscript text and Mariano Acosta prepared all the figures. All authors reviewed the manuscript.

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Correspondence to Maria Carolina Talio.

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Talio, M.C., Acosta, M. & Fernández, L.P. Determination of Lead in Bee Products by Solid Surface Fluorescence Using Complexation and Coacervation at Room Temperature Processes. An Environmental Friendly Methodology. J Fluoresc (2024). https://doi.org/10.1007/s10895-024-03622-y

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