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Photocatalytic, Antibacterial, Cytotoxic and Bioimaging Applications of Fluorescent CdS Nanoparticles Prepared in DNA Biotemplate

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Abstract

Synthesizing nanoparticles in biotemplates has been cited as one of the most promising way to obtain monodispersed inorganic nanoparticles. In this method, uniform voids in porous materials serve as hosts to confine the synthesized nanoparticles. DNA template can be described as a smart glue for assembling nanoscale building blocks. Here we investigate the photocatalytic, antibacterial, cytotoxic, and bioimaging applications of DNA capped CdS. XRD, SEM, TEM, UV–visible absorption, and photoluminescence spectra were used to study structural, morphological, and optical properties of CdS nanoparticles. Prepared CdS nanoparticles exhibit visible fluorescence. The photocatalytic activity of CdS towards Rhodamine 6G and Methylene blue are 64% and 91% respectively. A disc-diffusion method is used to demonstrate antibacterial screening. It was shown that CdS nanoparticles inhibit Gram-positive bacteria and Gram-negative bacteria effectively. DNA capped CdS shows higher activity than uncapped CdS nanoparticles. MTT cell viability assays were carried out in HeLa cells to investigate the cytotoxicity for 24 h. At a concentration 2.5 µg/ml, it shows 84% cell viability and 43% viability at 12.5 µg/ml. The calculated LC50 value is equal to 8 µg/ml. These DNA capped CdS nanoparticles were taken for an in-vitro experiment with HeLa cells to exhibit the possibility of bioimaging applications. The present study suggests that the synthesized CdS nanoparticles could be a potential photocatalyst, antibacterial agent, and biocompatible nanoparticle for bioimaging applications.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors would like to acknowledge CMET Thrissur; STIC CUSAT, Cochin; SAIF, MG University; CSIF, Calicut University; Central University of Kerala for the different studies of synthesized samples.

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Authors and Affiliations

  1. Photonic Materials Research Laboratory, Department of Physics, Government College Madappally, Vadakara, Kozhikode, 673102, Kerala, India

    V. N. Reena, G. S. Bhagyasree & B. Nithyaja

  2. University of Calicut, Malappuram, 673635, Kerala, India

    V. N. Reena, G. S. Bhagyasree & B. Nithyaja

  3. Department of Biochemistry and Molecular Biology, Central University of Kerala, Kasaragod, 671316, Kerala, India

    T. Shilpa & R. Aswati Nair

  4. Department of Physics, Baselius College, Kottayam, 686001, India

    H. Misha

  5. Mahatma Gandhi University, Kottayam, 686560, India

    H. Misha

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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Reena V N, Shilpa T, Aswati Nair R, Bhagyasree G S, Misha H and Nithyaja B. The first draft of the manuscript was written by Reena V N and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Reena, V.N., Bhagyasree, G.S., Shilpa, T. et al. Photocatalytic, Antibacterial, Cytotoxic and Bioimaging Applications of Fluorescent CdS Nanoparticles Prepared in DNA Biotemplate. J Fluoresc 34, 437–448 (2024). https://doi.org/10.1007/s10895-023-03292-2

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