Skip to main content
SpringerLink
Log in

Fluorimetric Reusable Polymeric Sensor for Hydrogen Sulfide Detection

  • RESEARCH
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

In this study, with the help of reactive monomers, crosslinkers, and photoinitiator that detect H2S in various matrices, an H2S sensitive fluorescence sensor polymerizes under ultraviolet (UV) light was developed. To this goal, a polymeric membrane was prepared, and the characterization of the membrane was carried out with Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. Afterward, appropriate conditions were identified, the excitation wavelength was determined as 370 nm, and the emission wavelength was determined as 425 nm. It was established that the fluorescence intensity of the prepared polymeric membrane decreased in the presence of H2S. A detailed analysis was executed to determine the sensor's most suitable pH value and time. It was found that the optimum pH was 8.0, and the optimal duration was 15 s. It has been calculated that the linear range of the developed method is 2.19 × 10–8– 6.25 × 10–7 M, and the detection limit (LOD) is 7.37 × 10–9 M. The effect of some possible interfering ions was investigated, and it determined that the sensor had excellent selectivity. In addition, the sensor used to determine H2S can be used at least 100 times. The recovery percentages were 102.1%–103.2%, and 104.6%, using tap water samples. In terms of providing reliable, fast results, high sensitivity, reusable, low cost, and ease of use, the developed fluorimetric sensor, compared to standard methods, has become more advantageous.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Data Availability

All data generated or analyzed during this study are included in this published article.

References

  1. Ayesh AI, Abu-Hani AFS, Mahmoud ST, Haik Y (2016) Selective H2S sensor based on CuO nanoparticles embedded in organic membranes. Sens Actuators B Chem 231:593–600. https://doi.org/10.1016/jsnb201603078

    Article  CAS  Google Scholar 

  2. Kimura H (2013) Physiological role of hydrogen sulfide and polysulfide in the central nervous system. Neurochem Int 63:492–497. https://doi.org/10.1016/j.neuint.2013.09.003

    Article  CAS  PubMed  Google Scholar 

  3. Barczak RJ, Możaryn J, Fisher RM, Stuetzb RM (2022) Odour concentrations prediction based on odorants concentrations from biosolid emissions. Environ Res 214:113871. https://doi.org/10.1016/j.envres.2022.113871

    Article  CAS  PubMed  Google Scholar 

  4. Taha A, Patón M, Rodríguez J (2022) Model-based design and operation of biotrickling filters for foul air H2S removal at wastewater networks. J Environ Chem Eng 10:107372. https://doi.org/10.1016/j.jece.2022.107372

    Article  CAS  Google Scholar 

  5. Spadaro L, Palella A, Frusteri F, Arena F (2015) Valorization of crude bio-oil to sustainable energy vector for applications in cars powering and on-board reformers via catalytic hydrogenation. Int J Hydrogen Energy 40:14507–14518. https://doi.org/10.1016/j.ijhydene.2015.08.010

    Article  CAS  Google Scholar 

  6. Yıldırım O, Kiss AA, Hüser N et al (2012) Reactive absorption in chemical process industry: a review on current activities. Chem Eng J 213:371–391. https://doi.org/10.1016/j.cej.2012.09.121

    Article  CAS  Google Scholar 

  7. Hittini W, Abu-Hani AF, Reddy N, Mahmoud ST (2020) Cellulose-Copper Oxide hybrid nanocomposites membranes for H2S gas detection at low temperatures. Nature 10:2940. https://doi.org/10.1038/s41598-020-60069-4

    Article  CAS  Google Scholar 

  8. Miyoshi J, Chang EB (2017) The gut microbiota and inflammatory bowel diseases. Transl Res 179:38–48. https://doi.org/10.1007/s00281-014-0454-4

    Article  CAS  PubMed  Google Scholar 

  9. Cao X, Xu H, Ding S et al (2016) Electrochemical determination of sulfide in fruits using alizarin-reduced graphene oxide nanosheets modified electrode. Food Chem 194:1224–1229. https://doi.org/10.1016/j.foodchem.2015.08.134

    Article  CAS  PubMed  Google Scholar 

  10. Berube PR, Parkinson PD, Hall ER (1999) Measurement of reduced sulphur compounds contained in aqueous matrices by direct injection into a gas chromatograph with a flame photometric detector. J Chromatogr A 830:485–489. https://doi.org/10.1016/S0021-9673(98)00882-6

    Article  CAS  Google Scholar 

  11. Colon M, Todoli JL, Hidalgo M et al (2008) Development of novel and sensitive methods for the determination of sulfide in aqueous samples by hydrogen sulfide generation-inductively coupled plasma-atomic emission spectroscopy. Anal Chim Acta 609:160–168. https://doi.org/10.1016/j.aca.2008.01.001

    Article  CAS  PubMed  Google Scholar 

  12. Huang R, Zheng X, Qu Y (2007) Highly selective electrogenerated chemiluminescence (ECL) for sulfide ion determination at multi-wall carbon nanotubes-modified graphite electrode. Anal Chim Acta 582:267–274. https://doi.org/10.1016/j.aca.2006.09.035

    Article  CAS  PubMed  Google Scholar 

  13. Hai Z, Bao Y, Miao Q et al (2015) Pyridine-biquinoline-metal complexes for sensing pyrophosphate and hydrogen sulfide in aqueous buffer and in cells. Anal Chem 87:2678–2684. https://doi.org/10.1021/ac504536q

    Article  CAS  PubMed  Google Scholar 

  14. Ma F, Sun M, Zhang K et al (2015) A turn-on fluorescent probe for selective and sensitive detection of hydrogen sulfide. Anal Chim Acta 879:104–110. https://doi.org/10.1016/j.aca.2015.03.040

    Article  CAS  PubMed  Google Scholar 

  15. Qian Y, Lin J, Liu T et al (2015) Living cells imaging for copper and hydrogen sulfide by a selective “on-off-on” fluorescent probe. Talanta 132:727–732. https://doi.org/10.1016/j.talanta.2014.10.034

    Article  CAS  PubMed  Google Scholar 

  16. Yuan Z-N, Zheng Y-Q, Wang B-H (2020) Prodrugs of hydrogen sulfide and related sulfur species: recent development. Chin J Nat Med 18:296–307. https://doi.org/10.1016/S1875-5364(20)30037-6

    Article  CAS  PubMed  Google Scholar 

  17. Wang K, Peng H, Ni N et al (2014) 2,6-Dansyl azide as a fluorescent probe for hydrogen sulfide. J Fluoresc 24:1–5. https://doi.org/10.1007/s10895-013-1296-5

    Article  CAS  PubMed  Google Scholar 

  18. Wang J, Yu H, Li Q et al (2025) A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells. Talanta 144:763–768. https://doi.org/10.1016/j.talanta.2015.07.026

    Article  CAS  Google Scholar 

  19. Huang K, Liu M, Liu Z et al (2015) Ratiometric and colorimetric detection of hydrogen sulfide with high selectivity and sensitivity using a novel FRET-based fluorescence probe. Dyes Pigm 118:88–94. https://doi.org/10.1016/j.dyepig.2015.03.007

    Article  CAS  Google Scholar 

  20. Olk R-M, Dietzsch W, Kahlmeier J et al (1997) Ligand exchange reactions between metal(II) chelates of different sulfur and selenium containing ligands X Exchange behavior of chelates of 1,3-dithiole-2-thione-4,5-diselenolate (dsit) and 1,1-dichalcogenolates X-ray structure of Bu4N[Pd(dsit) (Et2dsc)]. Inorganica Chim Acta 254:375–379. https://doi.org/10.1021/ja00980a017

    Article  CAS  Google Scholar 

  21. Chen X, Huang Z, Huang L et al (2022) Small-molecule fluorescent probes based on covalent assembly strategy for chemoselective bioimaging. RSC Adv 12:1393–1415. https://doi.org/10.1039/D1RA08037G

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Beyler-Cigil A, Danis O, Sarsar O et al (2021) Optimizing the immobilization conditions of β-galactosidase on UV-cured epoxy-based polymeric film using response surface methodology. J Food Biochem 45:13699. https://doi.org/10.1111/jfbc.13699

    Article  CAS  Google Scholar 

  23. Cubuk S, Taşci N, Kahraman MV (2016) Reusable fluorescent photocrosslinked polymeric sensor for determining lead ions in aqueous media. Spectrochim Acta A Mol Biomol Spectrosc 159:106–112. https://doi.org/10.1016/j.saa.2016.01.050

    Article  CAS  PubMed  Google Scholar 

  24. Li Z-Q, Zhou Z, Chen D-X et al (2022) Mitochondrial-targeted red-fluorescent chemodosimeter for hydrogen sulfide signaling and visualizing. Sens Actuators B Chem 369:132357. https://doi.org/10.1016/j.snb.2022.132357

    Article  CAS  Google Scholar 

  25. Jothi D, Iyer SK (2022) A highly sensitive naphthalimide based fluorescent “turn-on” sensor for H2S and its bio-imaging applications. J Photochem Photobiol 427:113802. https://doi.org/10.1016/j.jphotochem.2022.113802

    Article  CAS  Google Scholar 

  26. Wang L, Yang W, Song Y et al (2020) Novel turn-on fluorescence sensor for detection and imaging of endogenous H2S induced by sodium nitroprusside. Spectrochim Acta A Mol Biomol Spectrosc 243:118775. https://doi.org/10.1016/j.saa.2020.118775

    Article  CAS  PubMed  Google Scholar 

  27. El-Maghrabey MH, Watanabe R, Kishikawa N et al (2019) Detection of hydrogen sulfide in water samples with 2-(4-hydroxyphenyl)-4,5-di(2-pyridyl)imidazole-copper(II) complex using environmentally green microplate fluorescence assay method. Anal Chim Acta 1057:123–131. https://doi.org/10.1016/j.aca.2019.01.006

    Article  CAS  PubMed  Google Scholar 

  28. Ditrói T, Nagy A, Martinelli D et al (2019) Comprehensive analysis of how experimental parameters affect H2S measurements by the monobromobimane method. Free Radic Biol Med 136:146–158. https://doi.org/10.1016/j.freeradbiomed.2019.04.006

    Article  CAS  PubMed  Google Scholar 

  29. Coulomb B, Robert-Peillard F, Palacio E et al (2017) Fast microplate assay for simultaneous determination of thiols and dissolved sulfides in wastewater. Microchem J 132:205–210. https://doi.org/10.1016/j.microc.2017.01.022

    Article  CAS  Google Scholar 

  30. Xiang K, Liu Y, Li C (2015) A colorimetric and ratiometric fluorescent probe with a large Stokes shift for detection of hydrogen sulfide. Dyes Pigm 123:78–84. https://doi.org/10.1016/j.dyepig.2015.06.037

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chemistry Department, Faculty of Science, Marmara University, 34722, Istanbul, Türkiye

    Ayça Şeyma Ünaldı, Soner Çubuk & M. Vezir Kahraman

  2. Dep. of Chemistry and Chemical Process Technology School, Amasya University Technical Sci. Vocational, Amasya, Türkiye

    Aslı Beyler Çiğil

Authors
  1. Ayça Şeyma Ünaldı
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Soner Çubuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aslı Beyler Çiğil
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Vezir Kahraman
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

S.Ç.: Study design, Investigation, Preparation, Writing–original draft, Supervisor. A.Ş.Ü.: Photophysical measurements, Writing–original draft. A.B.Ç.: Photophysical measurements, Writing–original draft. M.V.K.: Study design, Investigation, Characterization. All authors reviewed the manuscript.

Corresponding author

Correspondence to Soner Çubuk.

Ethics declarations

Ethics Approval

Not applicable.

Consent to Participate

Not applicable.

Consent for Publication

Not applicable.

Conflicts of Interest

There are no conflicts to declare.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ünaldı, A.Ş., Çubuk, S., Çiğil, A.B. et al. Fluorimetric Reusable Polymeric Sensor for Hydrogen Sulfide Detection. J Fluoresc 33, 1651–1659 (2023). https://doi.org/10.1007/s10895-023-03181-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10895-023-03181-8

Keywords

Search

Navigation