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Highly Efficient Spectrophotometric Determination of Cu2+ ion in Aqueous Medium Using a thiosemicarbazone–derivative Ligand

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Abstract

A new simple spectrophotometric method for the determination of Cu2+ ion was developed using an thiosemicarbazone compound, 2-{4-[Bis(2-chloroethyl)amino]benzylidene}-N-[(4-methylthio)phenyl]hydrazinecarbothioamide (TSC). A simultaneous color change was observed (from colorless to bright yellow) by the addition of Cu2+ ion to the TSC ligand solution. The maximum absorbance of the TSC ligand measured at 366 nm was decreased by the presence of Cu2+ ion. The graphs of absorbance obtained by means of the Job’s method and the molar-ratio method proposed a complex formation with a 1:2 Cu2+–TSC ligand stoichiometry. The molar-ratio method with emission measurements also confirmed the stoichiometry. The complex stability constant of TSC–Cu2+ complex (K) was evaluated to be 1.76 × 105. The proposed spectrophotometric method was associated with the change in absorbance at 366 nm owing to the interaction between the TSC ligand and Cu2+ ion. From the spectrophotometric titration data, it was pointed out that TSC ligand (1.5 × 10− 5 mol L-1) selectively reacted with Cu2+ ion in DMSO/water (1:1, v/v, citrate buffer at pH = 6.0). The calibration curve for Cu2+ ion was obtained with a good linearity in the range of 0.0191–0.3241 mg L-1. The detection limit for Cu2+ ion was 0.0063 mg L-1. The proposed method was achievemently implemented in real water samples (drink water, tap water and, distilled water). Satisfactory recoveries were confirmed at three different concentrations. The method presented a relative standard deviation (RSD%) of less than 3.08%.

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Acknowledgements

We would like to thank Prof. Dr. Miraç OCAK for his consultancy in the application of fluorescence studies.

Funding

This research was supported by Scientific Research Foundation, Gümüşhane Universty, (19.A0118.02.01).

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Authors and Affiliations

  1. Department of Occupational Health and Safety, Faculty of Health Sciences, Gümüşhane University, 29100, Gümüşhane, Turkey

    Aysel BAŞOĞLU

  2. Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey

    Ümmühan OCAK

  3. Department of Chemistry, Faculty of Arts and Sciences, Gaziantep University, 27310, Gaziantep, Turkey

    Ayşegül KARAKÜÇÜK İYİDOĞAN

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  1. Aysel BAŞOĞLU
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Contributions

Aysel BAŞOĞLU and Ümmühan OCAK designed the study. Ayşegül KARAKÜÇÜK İYİDOĞAN synthesized and characterized the ligand. Aysel BAŞOĞLU prepared the manuscript and, performed all spectrophotometric/spectrofluorimetric measurements and, analysis. All authors read and approved the final manuscript.

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Correspondence to Aysel BAŞOĞLU.

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BAŞOĞLU, A., OCAK, Ü. & İYİDOĞAN, A.K. Highly Efficient Spectrophotometric Determination of Cu2+ ion in Aqueous Medium Using a thiosemicarbazone–derivative Ligand. J Fluoresc 33, 1003–1015 (2023). https://doi.org/10.1007/s10895-022-03127-6

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