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The Detection and Imaging of pH Values in Living Cells with Hemicyanine Based Colorimetric Mitochondria-Targeted Fluorescent Probe

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Abstract

pH plays a crucial role in cells, especially mitochondria, an important organelle. Developing probes with well-performance for pH detection is still in great demand. Therefore, we first synthesized an indole-based probe (MC-ID-OL) to detect mitochondrial pH changes. The emission wavelength of MC-ID-OL is 649 nm, which does not reach the near-infrared region (650–900 nm). To further enlarge the emission wavelength, probe MC-BI-OL was developed by replacing indolenine with benzoindole. As expected, the emission wavelength changed from 649 to 656 nm. MC-BI-OL probes could also detect pH changes and mitochondria's highly reversible proportional fluorescence localization. In addition, the fluorescence imaging of the MC-BI-OL in HeLa cells demonstrated that this probe could sense changes in the pH of mitochondria in cells.

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Data Availability Statement

The data that support this study are available in the article and accompanying online supplementary material.

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Funding

This work was supported by Two-Way Support Programs of Sichuan Agricultural University (Nos. 03572832 and 03572227).

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Authors and Affiliations

  1. College of Science, Sichuan Agricultural University, Ya’an, 625014, People’s Republic of China

    Zhengcheng Chen, Dan Wu, Han Yang, Ping Zou & Guangtu Wang

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  1. Zhengcheng Chen
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  2. Dan Wu
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  3. Han Yang
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  4. Ping Zou
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  5. Guangtu Wang
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Contributions

Zhengcheng Chen, Dan Wu and Han Yang completed the synthesis of the compound, the detection of related properties, and the writing of the article. Ping Zou and Guangtu Wang completed the revision and review of the article.

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Correspondence to Ping Zou or Guangtu Wang.

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Chen, Z., Wu, D., Yang, H. et al. The Detection and Imaging of pH Values in Living Cells with Hemicyanine Based Colorimetric Mitochondria-Targeted Fluorescent Probe. J Fluoresc 33, 393–399 (2023). https://doi.org/10.1007/s10895-022-03080-4

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