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Synthesis, Characterization and Applications of Schiff Base Chemosensor for Determination of Cr(III) Ions

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The development of a highly sensitive, selective, and efficient sensor for the determination and detection of Cr(III) ions remains a great challenge. Recently, some fluorescent chemosensors have been developed for the recognition of Cr(III) ions. But, the main drawbacks of the reported fluorescent chemosensors are the lack of selectivity and interference of anions and other trivalent cations. Herein, we designed and synthesized a novel thiazole-based fluorescent and colorimetric Schiff base chemosensor SB2 for the detection of Cr(III) ion by chemodosimetric approach. Using different analytical techniques including UV–vis, 13C-NMR, 1H-NMR, and FT-IR analysis the chemosensor SB2 was structurally characterized. The fully characterized chemosensor SB2 was used for the spectrofluorimetric and colorimetric detection of Cr(III) ions. Interestingly, chemosensor SB2 upon interaction with various metal cations including Ni2+, Na+, Cd2+, Ag+, Mn2+, K+, Zn2+, Cu2+, Hg2+, Co2+, Pb2+, Mg2+, Sn2+, Al3+ and Cr3+ displays highly selective and sensitive fluorescent (turn-on) and colorimetric (yellow to colorless) response toward Cr(III) ions. The fluorescence and UV–vis techniques confirmed the selective hydrolysis of azomethine group (-C = N-) of Schiff base chemosensor SB2 by Cr(III) ions. As a result, the fluorescence enhancement was observed that is corresponding to 2-hydroxy-1-nepthaldehyde (fluorophore). The chemosensor SB2 exhibits high interference performance towards Cr(III) ions over other metal cations in a wide pH range. Mover, the quite low detection limit was calculated to be 0.027 µg ml-1 (0.5 µM) (3σ/slop), lower than the maximum tolerable limits of Cr(III ions (10 µM) in drinking water permitted by the United States Environmental Protection Agency (EPA). These results show that chemosensor SB2 has great potential to detect selectively Cr(III) ions in the agricultural, environmental and biological analysis system.

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  1. Li C, Kräutler B (2015) Transition metal complexes of phyllobilins - a new realm of bioinorganic chemistry. Dalt Trans 44:10116–10127.

    Article  CAS  Google Scholar 

  2. Flemming CA, Trevors JT (1989) Copper toxicity and chemistry in the environment: a review. Water Air Soil Pollut 44:143–158.

    Article  CAS  Google Scholar 

  3. Festa RA, Thiele DJ (2011) Copper: An essential metal in biology. Curr Biol.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Sveshnikova EB, Ermolaev VL (2011) Inductive-resonant theory of nonradiative transitions in lanthanide and transition metal ions (review). Opt Spectrosc (English Transl Opt I Spektrosk) 111:34–50.

    Article  CAS  Google Scholar 

  5. Moustakas M (2021) The role of metal ions in biology, biochemistry and medicine. Materials (Basel) 14:1–4.

    Article  Google Scholar 

  6. Taghizadeh SF, Rezaee R, Badibostan H, Karimi G (2020) Probabilistic carcinogenic and non-carcinogenic risk assessment of heavy metal ingestion through consumption of different walnut cultivars: An Iranian study. Environ Monit Assess 192:1–15.

    Article  Google Scholar 

  7. Zuo TT, Li YL, He HZ, Jin HY, Zhang L, Sun L, Gao F, Wang Q, Shen YJ, Ma SC, He LC (2019) Refined assessment of heavy metal-associated health risk due to the consumption of traditional animal medicines in humans. Environ Monit Assess 191:1–12.

    Article  CAS  Google Scholar 

  8. Long Z, Huang Y, Zhang W, Shi Z, Yu D, Chen Y, Liu C, Wang R (2021) Effect of different industrial activities on soil heavy metal pollution, ecological risk, and health risk. Environ Monit Assess 193:1–12.

    Article  Google Scholar 

  9. Weissmannová HD, Pavlovský J (2017) Indices of soil contamination by heavy metals – methodology of calculation for pollution assessment (minireview). Environ Monit Assess 189:1–25.

    Article  Google Scholar 

  10. Cefalu WT, Hu FB (2004) Role of chromium in human health and in diabetes. Diabetes Care 27:2741–2751.

    Article  CAS  PubMed  Google Scholar 

  11. Dubey P, Thakur V, Chattopadhyay M (2020) Role of minerals and trace elements in diabetes and insulin resistance. Nutrients 12:1–17.

    Article  Google Scholar 

  12. Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN (2014) Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol 7:60.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ (2012) Heavy metal toxicity and the environment. EXS 101:133–164.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Tukur SA, Yusof NA, Hajian R (2015) Linear sweep anodic stripping voltammetry: Determination of Chromium (VI) using synthesized gold nanoparticles modified screen-printed electrode. J Chem Sci 127:1075–1081.

    Article  CAS  Google Scholar 

  15. Soylak M, Divrikli U, Saracoglu S, Elci L (2006) Membrane filtration – atomic absorption spectrometry combination for copper, cobalt, cadmium, lead and chromium in environmental samples. Environ Monit Assess 127:169–176.

    Article  PubMed  Google Scholar 

  16. Ma J, Wang Z, Li Q, Gai R, Li X (2014) On-line separation and preconcentration of hexavalent chromium on a novel mesoporous silica adsorbent with determination by solution-cathode glow discharge-atomic emission spectrometry. J Anal At Spectrom 29:2315–2322.

    Article  CAS  Google Scholar 

  17. Mokoena DP, Mngadi SV, Sihlahla M, Dimpe MK, Nomngongo PN (2019) Development of a rapid and simple digestion method of freshwater sediments for As, Cd, Cr, Cu, Pb, Fe, and Zn determination by Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES): An evaluation of dilute nitric acid. Soil Sediment Contam 28:323–333.

    Article  CAS  Google Scholar 

  18. Chen H, Du P, Chen J, Hu S, Li S, Liu H (2010) Separation and preconcentration system based on ultrasonic probe-assisted ionic liquid dispersive liquid–liquid microextraction for determination trace amount of chromium(VI) by electrothermal atomic absorption spectrometry. Talanta 81:176–179.

    Article  CAS  PubMed  Google Scholar 

  19. Timerbaev AR, Semenova OP, Buchberger W, Bonn GK (1996) Speciation studies by capillary electrophoresis- Simultaneous determination of chromium(III) and chromium(VI), Fresenius’. J Anal Chem 354:414–419.

    Article  CAS  Google Scholar 

  20. Ying LY, Jiang HL, Zhou SC, Zhou Y (2011) Ionic liquid as a complexation and extraction medium combined with high-performance liquid chromatography in the evaluation of chromium(VI) and chromium(III) speciation in wastewater samples. Microchem J 98:200–203.

    Article  CAS  Google Scholar 

  21. Martínez-Gallegos S, Bulbulian S (2005) Neutron activation analysis for chromium(III) and (VI) in lixiviated liquid through a calcined chromium(VI) adsorbed hydrotalcite. J Radioanal Nucl Chem 266:285–287.

    Article  Google Scholar 

  22. Nural Y, Keleş E, Aydıner B, Seferoğlu N, Atabey H, Seferoğlu Z (2021) New naphthoquinone-imidazole hybrids: Synthesis, anion recognition properties, DFT studies and acid dissociation constants. J Mol Liq 327, p.114855.

  23. Keleş E, Aydıner B, Nural Y, Seferoğlu N, Şahin E, Seferoğlu Z (2020) A new mechanism for selective recognition of cyanide in organic and aqueous solution. European J Org Chem 2020:4681–4692.

    Article  Google Scholar 

  24. Khan S, Chen X, Almahri A, Allehyani ES, Alhumaydhi FA, Ibrahim MM, Ali S (2021) Recent developments in fluorescent and colorimetric chemosensors based on schiff bases for metallic cations detection: A review. J Environ Chem Eng 9:106381.

    Article  CAS  Google Scholar 

  25. Muhammad M, Khan S, Fayaz H (2021) Charge-transfer complex–based spectrophotometric method for the determination of mesotrione in environmental samples. Environ Monit Assess 193:1–7.

  26. Gul Z, Ullah S, Khan S, Ullah H, Khan MU, Ullah M, Ali S, Altaf AA (2022) Recent progress in nanoparticles based sensors for the detection of mercury (II) ions in environmental and biological samples. Crit Rev Anal Chem 52:1–17.

  27. Al-Saidi HM, Khan S (2022) Recent advances in Thiourea based colorimetric and fluorescent chemosensors for detection of anions and neutral analytes: a review. Crit Rev Anal Chem 52:1–17.

  28. Abd-Elzaher MM, Labib AA, Mousa HA, Moustafa SA, Ali MM, El-Rashedy AA (2016) Synthesis, anticancer activity and molecular docking study of Schiff base complexes containing thiazole moiety, Beni-Suef Univ. J Basic Appl Sci 5:85–96.

    Article  Google Scholar 

  29. Mergu N, Gupta VK (2015) A novel colorimetric detection probe for copper(II) ions based on a Schiff base. Sensors Actuators B Chem 210:408–417.

    Article  CAS  Google Scholar 

  30. Li X, Zhang S, Dang Y, Liu Z, Zhang Z, Shan D, Zhang X, Wang T, Lu X (2019) Ultratrace naked-eye colorimetric ratio assay of chromium(III) ion in aqueous solution via stimuli-responsive morphological transformation of silver nanoflakes. Anal Chem 91:4031–4038.

    Article  CAS  PubMed  Google Scholar 

  31. Al-Hazmi GAA, Abou-Melha KS, El-Metwaly NM, Althagafi I, Shaaban F, Elghalban MG, El-Gamil MM (2020) Spectroscopic and theoretical studies on Cr (III), Mn (II) and Cu (II) complexes of hydrazone derived from picolinic hydrazide and O-vanillin and evaluation of biological potency. Appl Organomet Chem 34:e5408.

    Article  CAS  Google Scholar 

  32. Panhwar QK, Memon S (2014) Synthesis of Cr (III)-morin complex: Characterization and antioxidant study. Sci World J 2014:8–13.

  33. Prabhu D, Laxmeshwar NB (1997) Effect of Transition Metal Ions on the Hydrolysis of the Methyl Derivatives of Salicylanil. Asian J Chem 9:70–74

  34. Huang M, Zhou J, Zheng X, Zhang Y, Xu S, Li Z (2020) Novel spiropyran derivative based reversible photo-driven colorimetric and fluorescent probes for recognizing Fe3+, Cr3+ and Al3+ metal ions. Inorg Chem Commun 117:107968.

  35. Tajbakhsh M, Chalmardi GB, Bekhradnia A, Hosseinzadeh R, Hasani N, Amiri MA (2018) A new fluorene-based Schiff-base as fluorescent chemosensor for selective detection of Cr3+ and Al3+, Spectrochim. Acta - Part A Mol Biomol Spectrosc 189:22–31.

    Article  CAS  Google Scholar 

  36. Kumawat LK, Mergu N, Asif M, Gupta VK (2016) Novel synthesized antipyrine derivative based “naked eye” colorimetric chemosensors for Al3+ and Cr3+. Sensors Actuators, B Chem 231:847–859.

    Article  Google Scholar 

  37. Zhu W, Yang L, Fang M, Wu Z, Zhang Q, Yin F, Huang Q, Li C (2015) New carbazole-based Schiff base: Colorimetric chemosensor for Fe3+ and fluorescent turn-on chemosensor for Fe3+ and Cr3+. J Lumin 158:38–43.

    Article  CAS  Google Scholar 

  38. Saluja P, Sharma H, Kaur N, Singh N, Jang DO (2012) Benzimidazole-based imine-linked chemosensor: Chromogenic sensor for Mg2+ and fluorescent sensor for Cr3+. Tetrahedron 68:2289–2293.

    Article  CAS  Google Scholar 

  39. Zheng XY, Zhang WJ, Mu L, Zeng X, Xue SF, Tao Z, Yamatob T (2010) A novel rhodamine-based thiacalix[4]arene fluorescent sensor for Fe3+ and Cr3+. J Incl Phenom Macrocycl Chem 68:139–146.

    Article  CAS  Google Scholar 

  40. Feng S, Gao Z, Liu H, Huang J, Li X, Yang Y (2019) Feasibility of detection valence speciation of Cr(III) and Cr(VI) in environmental samples by spectrofluorimetric method with fluorescent carbon quantum dots, Spectrochim. Acta Part A Mol Biomol Spectrosc 212:286–292.

    Article  CAS  Google Scholar 

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Authors and Affiliations



Sikandar Khan: Writing Original Draft-Equal, Conceptualization-Equal, Mian Muhammad: Supervision-Equal, Data Curation-Equal, Hamed M. Al-Saidi: Formal analysis-Equal, Athar Abdulfattah Hassanian: Visualization-Equal, Jari S. Algethami: Editing-Equal, Albandary Almahri: Data Curation-Equal, Editing-Equal.

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Correspondence to Sikandar Khan or Mian Muhammad.

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Khan, S., Muhammad, M., Algethami, J.S. et al. Synthesis, Characterization and Applications of Schiff Base Chemosensor for Determination of Cr(III) Ions. J Fluoresc 32, 1889–1898 (2022).

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