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Development of Optical Biosensor for the Detection of Glutamine in Human Biofluids Using Merocyanine Dye

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Abstract

Merocyanine dye based fluorescent organic compound has been synthesized for the detection of glutamine. The probe showed remarkable fluorescent intensity with glutamine through ICT (Intermolecular Charge Transfer Mechanism). Hence, it is tested for the detection of glutamine using colorimetric and fluorimetric techniques in physiological and neutral pH (7.2). Under optimized experimental conditions, the probe detects glutamine selectively among other interfering biomolecules. The probe has showed a LOD (lower limit of detection) of 9.6 × 10–8 mol/L at the linear range 0–180 µM towards glutamine. The practical application of the probe is successfully tested in human biofluids.

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Acknowledgements

The author VS acknowledged DST, New Delhi for the INSPIRE fellowship (IF180132). Authors thank for financial support under DST-IRHPA, FIST, RUSA-MKU and PURSE for instrument facilities.

Funding

The authors received no specific funding for this work.

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Authors and Affiliations

  1. Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamilnadu, India

    Vijayakumar Sathya, Appadurai Deepa, Lakshmi Kandhan Sangeetha & Vediappen Padmini

  2. Division of Chemistry, Department of Sciences and Humanities, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur, 522 213, Andhra Pradesh, India

    Venkatesan Srinivasadesikan

  3. Department of Chemistry and Biochemistry, National Chung-Cheng University, Chia-Yi, 621, Taiwan

    Shyi-Long Lee

Authors
  1. Vijayakumar Sathya
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  2. Appadurai Deepa
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  3. Lakshmi Kandhan Sangeetha
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  4. Venkatesan Srinivasadesikan
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  5. Shyi-Long Lee
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  6. Vediappen Padmini
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Contributions

Vijayakumar Sathya: Conceptualization, Writing-original draft. Appadurai Deepa: Revising the article. Lakshmi Kandhan Sangeetha: Revising the article. Venkatesan Srinivasadesikan: Software resources. Shyi-Long Lee: Software resources. Vediappen Padmini: Supervision, Writing-review.

Corresponding author

Correspondence to Vediappen Padmini.

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Highlights

• The organic sensor of merocyanine dye was synthesized by a simple condensation method with moderate yield.

• The dye as a sensor detects the glutamine with high fluorescence intensity.

• The sensor was selectively detects glutamine while interfering other biomolecules and biologically important metals.

Supplementary Information

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Supplementary file1 (DOCX 6454 KB)

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Sathya, V., Deepa, A., Sangeetha, L.K. et al. Development of Optical Biosensor for the Detection of Glutamine in Human Biofluids Using Merocyanine Dye. J Fluoresc 32, 1389–1396 (2022). https://doi.org/10.1007/s10895-022-02937-y

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  • DOI: https://doi.org/10.1007/s10895-022-02937-y

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