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Effect of Temperature on Re-entrant Condensation of Globular Protein in Presence of Tri-valent Ions

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Abstract

Globular proteins play several essential roles in functioning different mechanisms of the living organisms, and the stability of such protein molecules in an aqueous solution is strongly affected by multivalent ions. In this article, we have systematically studied the effect of temperature (between 5 and 25ºC) on the re-entrant condensation behaviour of bovine serum albumin (BSA) in the presence of trivalent ions, Yttrium (Y3+), and Lanthanum (La3+). The effect of temperature is explained considering the optical properties of the protein, i.e., from the optical absorption and emission behaviours. The absorption in the visible region and the fluorescence emission of BSA becomes maximum at the lowest temperature. The decrement of mobility at lower temperature is responsible for fluorescence enhancement. Moreover, the activation energy of the turbid or viscus phase of the BSA protein under re-entrant condensation is enhanced in comparison with the transparent phase and the corresponding energy value is estimated from the fluorescence study.

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Acknowledgements

S. Pandit acknowledges Department of Science and Technology, Govt. of India for the financial support through INSPIRE Fellowship (IF 160402). S. Kundu acknowledges financial support from Department of Science and Technology, Govt. of India. Authors acknowledge IASST, Guwahati for the financial and other experimental facilities.

Funding

SP obtained the financial support through INSPIRE Fellowship (Grant no. IF 160402) supported by Department of Science and Technology, Govt. of India. SK obtained the financial support from Department of Science and Technology, Govt. of India.

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  1. Soft Nano Laboratory, Physical Sciences Division, Institute of Advanced Study in Science and Technology, Vigyan Path, Paschim Boragaon, Garchuk, Guwahati, Assam, 781035, India

    Subhankar Pandit & Sarathi Kundu

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  1. Subhankar Pandit
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  2. Sarathi Kundu
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SP and SK conceptualized the study and designed the experiments. SP performed all the experiments and wrote the manuscript. SK supervised the work and approved the manuscript.

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Correspondence to Sarathi Kundu.

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Pandit, S., Kundu, S. Effect of Temperature on Re-entrant Condensation of Globular Protein in Presence of Tri-valent Ions. J Fluoresc 32, 791–797 (2022). https://doi.org/10.1007/s10895-021-02874-2

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