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A Facile Probe for Fluorescence Turn-on and Simultaneous Naked-Eyes Discrimination of H2S and biothiols (Cys and GSH) and Its Application

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Abstract

Hydrogen sulfide and biothiol molecules such as Cys and GSH acted important roles in many physiological processes. To simultaneously detect and distinguish them was quite necessary by a suitable fluorescent probe. A novel chemosensor 4-(4-(benzo[d]thiazol-2-yl)-2-methoxyphenoxy)-7-nitrobenzo[c][1,2,5]oxadiazole (BMNO) was designed to detect H2S/Cys/GSH using the combination of nitrobenzofurazan (NBD) and benzothiazole fluorophores linked by a facile ether bond. The probe BMNO was developed for simultaneous identification of H2S, Cys and GSH. Noticeably, the color changes (from colorless to light purple, light orange and light yellow) of probe BMNO solutions for sensing H2S, Cys and GSH could be observed by naked eyes, respectively. The probe BMNO exhibited high selectivity and sensitivity for H2S, Cys and GSH showing distinct optical signal with detection limit as low as 0.15 μM, 0.03 μM and 0.14 μM, respectively. The sensing mechanism was clarified by spectrum analysis and some controlled experiments. In addition, these outstanding properties of probe BMNO enabled its practical applications in detection H2S in beer, and in cell imaging for Cys and GSH as well.

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Acknowledgements

This work was supported by the Students' Innovation and Entrepreneurship Training Program of Northeast Agricultural University (No. 202010224124).

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  1. Ting-Ting Liu and Shi-Jie Li contributed equally to this work

Authors and Affiliations

  1. School of Laboratory Medicine, Youjiang Medicinal University for Nationality, Baise, 533000, Guangxi, China

    Zhi-Yong Xing

  2. Department of Chemistry, College of Art and Sciences, Northeast Agricultural University, Harbin, 150030, PR China

    Ting-Ting Liu, Xiao-Song Zhang, Jing Wang, Xue-Jiao Sun & Ruo-Fei Wu

  3. College of Life Science, Northeast Agricultural University, Harbin, 150030, PR China

    Shi-Jie Li

  4. Qingdao Huamao Construction Development Co, Led, Qingdao, 266000, PR China

    Yun-Xiu Deng

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Contributions

T.T.L writing-original draft and visualization; S.J.L methodology and formal analysis; X.J.S synthesis and data curation; J.W. and Y.X.D Validation; Z.Y.X conceptualization, methodology, and writing-review & editing; X.S.Z. and R.F.W. equipment’s and laboratory facilities.

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Liu, TT., Li, SJ., Zhang, XS. et al. A Facile Probe for Fluorescence Turn-on and Simultaneous Naked-Eyes Discrimination of H2S and biothiols (Cys and GSH) and Its Application. J Fluoresc 32, 175–188 (2022). https://doi.org/10.1007/s10895-021-02838-6

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