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Differences in Spectroscopic Properties of Saliva Taken From Normal Subjects and Oral Cancer Patients: Comparison Studies

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Abstract

Oral cancer disease is among the most common cancers in the world and are associated with mortality and morbidity. The characterization of saliva samples may help to distinguish patients with oral cancer disease from normal subjects. To characterize spectra of saliva samples from normal subjects and oral cancer patients by use of fluorescence, absorption, and 1H-NMR spectroscopy. Whole unstimulated saliva samples were collected from patients with oral cancer disease and normal subjects. The saliva samples were analyzed by absorption, fluorescence and 1H-NMR spectroscopic techniques. The characteristic spectra of saliva samples from patients with oral cancer disease and normal subjects were compared. For fluorescence spectroscopic studies, six fluorophores were found in saliva samples. Autofluorescence emission spectra and synchronous spectra of saliva were different between normal subjects and oral cancer patients. For absorption spectroscopic studies, the typical absorption spectra of saliva samples from normal subjects and oral cancer patients were also different in absorption intensity, 1st and 2nd derivative of absorption spectra values. For 1H-NMR studies, nine metabolites and four metabolites were found in saliva samples taken from normal subjects and oral cancer patients, respectively. The metabolic profiles of saliva samples from normal subjects and oral cancer patients were not similar. The characteristic spectra of saliva samples from normal subjects and oral cancer patients were found. These results showed differences in the spectra of saliva samples between both that groups. The spectra from each spectroscopic techniques could determine a candidate saliva biomarkers for distinguishing patients with oral cancer disease from normal subjects.

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Acknowledgements

Khin TheNu Aye would like to thank the Ph.D. degree program in biomedical sciences, Faculty of Associated Medical Sciences, Chiang Mai University, under the CMU Presidential Scholarship. The authors would like to thank the Faculty of Associated Medical Sciences, Chiang Mai University and Lampang Cancer Hospital, Lampang, Thailand for their support. The authors also would like to thank Pranruethai Chaimongkol and Chadaporn Thaithiang for their participation.

Funding

This research was partially supported by Chiang Mai University. This study was also supported by the Faculty of Associated Medical Sciences, Chiang Mai University.

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Authors and Affiliations

  1. Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand

    Benjamaporn Supawat, Khin TheNu Aye, Suchart Kothan & Montree Tungjai

  2. Center of Radiation Research and Medical Imaging, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand

    Benjamaporn Supawat, Khin TheNu Aye, Suchart Kothan, Jie Pan & Montree Tungjai

  3. Lampang Cancer Hospital, Lampang, 52000, Thailand

    Janejirarak Ritpanja & Wiphaporn Nueangwong

  4. Ph.D. degree program in biomedical sciences, Faculty of Associated Medical Sciences, Chiang Mai University, under the CMU Presidential Scholarship, Chiang Mai, 50200, Thailand

    Khin TheNu Aye

  5. Shandong Provincial Key Laboratory of Animal Resistant Biology, College of Life Sciences, Shandong Normal University, Jinan, China

    Jie Pan

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  1. Benjamaporn Supawat
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Contributions

BS. and KTNA. ; acquisition and analysis of data. JR. and WN. ; sample collection., SK.; acquisition, analysis, interpretation of data and provided critical discussions. JP.; interpretation of data and provided critical discussions. MT.; study conception and design, acquisition, analysis, and interpretation of data, provided critical discussions, drafting the manuscript and writing of the manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Montree Tungjai.

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The present study was approved by the Ethics Committee of the Faculty of Associated Medical Sciences, Chiang Mai University, Chaing Mai, Thailand (No. AMSEC-63EX-012). 

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Supawat, B., Aye, K.T., Ritpanja, J. et al. Differences in Spectroscopic Properties of Saliva Taken From Normal Subjects and Oral Cancer Patients: Comparison Studies. J Fluoresc 31, 747–754 (2021). https://doi.org/10.1007/s10895-021-02707-2

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