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Investigation of Gd2O3: Er3+/Yb3+ Upconversion Nanoparticles (UCNPs) as a Multi‐model Contrast Agent for Functional Optical Coherence Tomography (fOCT)

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Abstract

Currently, upconversion nanoparticles (UCNPs) implanted as a contrast agent for optical coherence tomography (OCT) system due to its biocompatibility, anti-stock emission, narrow emission bandwidth non-photobleaching effects etc., but it was not used as multi model imaging probe. We synthesized multimodal imaging probe having upconversion property along with paramagnetic property and used as dual contrast agents for Photothermal Optical Coherence Tomography (PTOCT) and Magnetomotive Optical Coherence Tomography (MMOCT). The synthesized Gd2O3:Er3+/Yb3+ UCNPs shows the bright yellow upconversion emission, biocompatibility with hydrophilic property. A custom built SSOCT setup modified for PTOCT and MMOCT imaging along with custom MATLAB algorithm for signal extraction. A dynamic study was performed with synthesized UCNPs as an imaging probe and functional OCT system for targeted imaging. This shows the utility of the Gd2O3:Er3+/Yb3+ UCNPs as molecular probe for targeted imaging applications.

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Data will be made available on reasonable request.

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Funding

This research is supported by DST-SERB (EMR/2015/001757) Govt. of India and Indian Council of Medical Research (ICMR) via grant no. ICMR-EMR (5/2021 (Bio)/2014-ITR-1).

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Authors and Affiliations

  1. Biophotonics Lab, Department of Bioengineering, Birla Institute of Technology-Mesra, Ranchi, JH, 835215, India

    Muktesh Mohan & Raju Poddar

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  1. Muktesh Mohan
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  2. Raju Poddar
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Contributions

MM and RP developed the theoretical formalism, performed the experiments and analytic calculations. Both MM and RP authors contributed to the final version of the manuscript. RP supervised the project.

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Correspondence to Raju Poddar.

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Cell Lines were used namely, Human Cervical Cancer (HeLa: RRID:CVCL_1D61) and Human Embryonic Kidney 293 (HEK293: RRID:CVCL_S025). 

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Mohan, M., Poddar, R. Investigation of Gd2O3: Er3+/Yb3+ Upconversion Nanoparticles (UCNPs) as a Multi‐model Contrast Agent for Functional Optical Coherence Tomography (fOCT). J Fluoresc 31, 541–550 (2021). https://doi.org/10.1007/s10895-021-02681-9

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  • DOI: https://doi.org/10.1007/s10895-021-02681-9

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