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In Vitro Targeting of NL2 Peptide Bounded on Poly L-DOPA Coated Graphene Quantum Dot

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Abstract

Chemotherapy using drug delivery systems (DDS) can target cancer cells selectively and without affecting normal cells. In this paper, NL2 peptide as a tumor targeted peptide was bonded on the surface of poly 3,4-Dihydroxy-L-phenylalanine (Poly L-DOPA) graphene quantum dots (GQD), which was imprinted by Doxorubicin (DOX). The synthesized nanocomposite was characterized by Fourier-transform infrared spectroscopy (FTIR) and particle size was determined by dynamic light scattering (DLS) and Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). DOX release from synthesized nano-composite was investigated spectrophotometrically. Also, the toxicity and selectivity of NL2-GQD-NC on SK-BR-3 cell line were evaluated. FTIR and DLS experiment confirm the successful synthesis of Poly L-DOPA coated graphene quantum dots and their uniform particles. In vitro studies have shown that NL2-GQD-NC attached more to SK-BR-3 cells than NL2-free nanocomposites (GQD-NC). After attaching the cells could be imaged due to the presence of GQD particles and DOX release was accomplished in the tumor cells.

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The authors have no any objection for availability of data and materials.

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Acknowledgments

We thank the staff of the Semnan University of Medical sciences, Drug Design and Bioinformatics Unit and Biotechnology Research Center from Pasteur Institute of Iran.

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Authors and Affiliations

  1. Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Mahdi Mirzababaei, Kambiz Larijani & Zohreh Mirjafary

  2. Department of Medicinal Chemistry, School of Pharmacy, Damghan Branch, Islamic Azad University, Damghan, Iran

    Hamid Hashemi-Moghaddam

  3. Department of Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

    Hamid Madanchi

  4. Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Hamid Madanchi

Authors
  1. Mahdi Mirzababaei
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  2. Kambiz Larijani
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  3. Hamid Hashemi-Moghaddam
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  4. Zohreh Mirjafary
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  5. Hamid Madanchi
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Contributions

All authors contributed in the present study. All authors commented on previous version of the manuscript and they read and approved the final manuscript.

Corresponding author

Correspondence to Hamid Hashemi-Moghaddam.

Ethics declarations

The authors did not receive support from any organization for the submitted work. The authors have no relevant financial or non-financial interests to disclose. All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this manuscript. Conflict of interest Authors declare that they have no conflict of interest.

Human Studies

The present research does not involve human participants, their data or biological material. The study was carried out in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki) for experiments involving humans and was approved by the ethics committee of the Islamic Azad University Science and research branch.IR.IAU.SRB.1398.104.

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Mirzababaei, M., Larijani, K., Hashemi-Moghaddam, H. et al. In Vitro Targeting of NL2 Peptide Bounded on Poly L-DOPA Coated Graphene Quantum Dot. J Fluoresc 31, 279–288 (2021). https://doi.org/10.1007/s10895-020-02660-6

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  • DOI: https://doi.org/10.1007/s10895-020-02660-6

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