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Selective Fluorometric Analysis of Hg(II) in Industrial Waste Water Samples

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Abstract

The highly selective and sensitive fluorometric method has been developed for trace level determination of Hg(II) is based on photo-induced electron transfer between rhodamine-6G dye and metal complex. Quenching in fluorescence intensity by fluorescence resonance energy transfer (FRET) is due to interaction between metal ion complex and dye. The fluorescence emitted was measured at 510 and 550 nm, for excitation and emission wavelengths respectively. Possible interferences present in water samples, which could affect the analytical response are studied and determined. The calibration graph was dynamically linear from 0.002 to 0.05 mgL−1 of Hg(II) with limit of detection 7 × 10−4 mgL−1 and limit of quantitation 1.9 × 10−3 mgL−1. The Stern-Volmer constant (KSV) calculated for the quenching of R-6G with Hg (II) was 8.47 Lmg−1 s−1 at optimized reaction conditions. The proposed FRET based fluorometric method was applied successfully in different industrial wastewater samples with satisfactory outcome.

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Acknowledgments

We acknowledge gratefully to National Institute of Technology, Raipur (CG) India for making availability of essential instrument as well as lab facility. We sincerely show our gratitude to University Grants Commission (UGC), New Delhi, India for providing financial endorsement as fellowship (JRF student id-141394).

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  1. Department of Chemistry, National Institute of Technology, Raipur, CG, India

    Saurabh Kumar Gupta, Kavita Tapadia & Ashima Sharma

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  1. Saurabh Kumar Gupta
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Correspondence to Kavita Tapadia.

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Gupta, S.K., Tapadia, K. & Sharma, A. Selective Fluorometric Analysis of Hg(II) in Industrial Waste Water Samples. J Fluoresc 30, 1375–1381 (2020). https://doi.org/10.1007/s10895-020-02627-7

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