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Detection of Malachite Green in Water Using Edge Excited Label Free Fluorescent Probe NCQDs

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Abstract

The fluorescent properties of nitrogen doped carbon quantum dots (NCQDs) prepared through microwave assisted green method has been used as label free fluorescent probe for selective and sensitive detection of malachite green (MG) in water. The optical responses revealed that the NCQDs are highly stable and have good fluorescent quantum yield. The NCQDs were used to detect the Malchite Green in Mili Q water. Reduction in the fluorescence response was monitored in the range 17.12–128.43 μM of MG dissolved in Mili Q water. Linear response was observed in the range, 10–80 μM. The calculated value of limit of detection is 5.16 μM and the sensitivity is (0.03536 ± 0.00001) μM−1. The future application of this work is that it can be employed to detect MG in the tap water and other natural sources of water.

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References

  1. Moumeni O, Hamdaoui O (2012) Intensification of sonochemical degradation of malachite green by bromide ions. Ultrason. Sonochem. 19: 404–409

  2. Ray SK, Dhakal D, Lee SW (2017) Insight into malachite green degradation, mechanism and pathways by morphology-tuned a-NiMoO4 photocatalyst, photochemistry and photobiology

  3. Ju Y, Yang S, Ding Y, Sun C, Zhang A, Wang L (2008) Microwave-assisted rapid photocatalytic degradation of malachite green in TiO2 suspensions: mechanism and pathways. J Phys Chem A 112:11172–11177

    Article  CAS  Google Scholar 

  4. Aliyan H, Fazaeli R, Jalilian R (2013) Fe3O4@mesoporous SBA-15: a magnetically recoverable catalyst for photo degradation of malachite green. Appl Surf Sci 276:147–153

    Article  CAS  Google Scholar 

  5. Yanli Z, Xiaoqiao L, Zhonghao P, Baoxian Y, Maotian X (2019) Determination of malachite green in fish by a modified mof-based electrochemical sensor. Food Analytical Methods 12:1246–1254

    Article  Google Scholar 

  6. Srivastava S, Sinha R, Roy D (2004) Toxicological effects of malachite green. Aquat Toxicol 66:319–329

    Article  CAS  Google Scholar 

  7. Fallah AA, Barani A (2014) Determination of malachite green residues in farmed rainbow trout in Iran. Food Control 40:100–105

    Article  CAS  Google Scholar 

  8. Chen GY, Miao S (2010) HPLC determination and MS confirmation of malachite green, gentian violet, and their leuco metabolite residues in channel catfish muscle. J Agric Food Chem 58:7109–7114

    Article  CAS  PubMed  Google Scholar 

  9. Maxwelland EJ, Tong WG (2016) Sensitive detection of malachite green and crystal violet by nonlinear laser wave mixing and capillary electrophoresis. J Chromatogr B 1020:29–35

    Article  Google Scholar 

  10. Wang HZ, Wang Y, Liu S, Yu JH, Xu W, Guoand YN, Huang JD (2014) An RNA aptamer-based electrochemical biosensor for sensitive detection of malachite green. RSC Adv 4:60987–60994

    Article  CAS  Google Scholar 

  11. Wang YL, Liao KR, Huangand XJ, Yuan DX (2015) Simultaneous determination of malachite green, crystal violet and their leuco-metabolites in aquaculture water samples using monolithic fiber-based solid phase micro extraction coupled with high performance liquid chromatograph. Anal Methods 7:8138–8145

    Article  CAS  Google Scholar 

  12. Wang F, Wang H, Shen YD, Li YJ, Dong JX, Xu ZL, Yang JY, Sunand YM, Xiao ZL (2016) Bi-specific monoclonal antibody-based multi-analyte ELISA for furaltadone metabolite, malachite green and leucomalachite green in aquatic products. J Agric Food Chem 64:8054–8061

    Article  CAS  PubMed  Google Scholar 

  13. Dong JX, Xu C, Wang H, Xiao ZL, Gee SJ, Li ZF, Wang F, Wu WJ, Shen YD, Yang JY, Sun YM, Hammock BD (2014) Enhanced sensitive immunoassay: noncompetitive phage anti-immune complex assay for the determination of malachite green and leucomalachite green. J Agric Food Chem 62:8752–8758

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Xie J, Peng T, Chen DD, Zhang QJ, Wang GM, Wang X, Guo Q, Jiang F, Chenand D, Deng J (2013) Determination of malachite green, crystal violet and their leuco-metabolites in fish by HPLC–VIS detection after immune affinity column clean-up. J Chromatogr B 913–914 123–128

  15. Guo ZY, Gai PP, Hao TT, Duan J, Wang S (2011) Determination of malachite green residues in fish using a highly sensitive electrochemiluminescence method combined with molecularly imprinted solid phase extraction. J Agric Food Chem 59:5257–5262

    Article  CAS  PubMed  Google Scholar 

  16. Liu LT, Zhou YL, Liuand S, Xu MT (2018) The applications of metal-organic frameworks in electrochemical sensors. Chem Electro Chem 5:6–19

    CAS  Google Scholar 

  17. Singh R, Kashyap S, Kumar S, Abraham S, Gupta TK, Kayastha AM, Malhotra BD, Saxena PS, Srivastava A, Singh RK (2017) Excellent storage stability and sensitive detection of neurotoxin quinolinic acid. Biosens Bioelectron 90:224–229

    Article  CAS  PubMed  Google Scholar 

  18. Laviron E (1974) Study on the behavior of the mercury film electrode. J Electroanal Chem 52:355–393

    Article  CAS  Google Scholar 

  19. Wu L, Lin ZZ, Zhong HP, Chen XM, Huang ZY (2017) Rapid determination of malachite green in water and fish using a fluorescent probe based on CdTe quantum dots coated with molecularly imprinted polymer. Sensor Actuators B Chem 239:69–75

    Article  CAS  Google Scholar 

  20. Singh R, Rai SK, Tiwari MK, Mishra A, Tewari AK, Mishra PC, Singh RK (2017) An excellent stable fluorescent probe: selective and sensitive detection of trace amounts of hg+2 ions in natural source of water. Chem Phys Lett 676:39–45

    Article  CAS  Google Scholar 

  21. Singh R, Kashayap S, Singh V, Kayastha AM, Mishra H, Saxena PS, Srivastava A, Singh RK (2018) QPRTase modified N-doped carbon quantum dots: a fluorescent bioprobe for selective detection of neurotoxin quinolinic acid in human serum. Biosens Bioelectron 101:103–109

    Article  CAS  PubMed  Google Scholar 

  22. Singh AK, Prakash P, Singh R, Nandy N, Firdaus Z, Bansal M, Singh RK, Srivastava A, Roy JK, Mishra B, Singh RK (2017) Curcumin quantum dots mediated degradation of bacterial biofilms. Front Microbiol 8:1517

    Article  PubMed  PubMed Central  Google Scholar 

  23. Singh R, Yadav A, Shekhar S, Ajad RK, Singh RK, Kayastha AM (2019) Nitrogen doped carbon quantum dots modified by Lens culinaris β-Galactosidase as a fluorescent probe for detection of lactose. J Fluoresc 29:1213–1219

    Article  CAS  PubMed  Google Scholar 

  24. Singh R, Mishra VK, Singh HK, Sharma G, Koch B, Singh B, Singh RK (2018) Mg co-ordination with potential carcinogenic molecule acrylamide: Spectroscopic, computational and cytotoxicity studies. J. Mol. Struct 1155:330–336

    Article  CAS  Google Scholar 

  25. Singh R, Tiwari MK, Gangopadhyay D, Mishraa PC, Mishra H, Srivastava A, Singh RK (2018) Detection and monitoring of in vitro formation of salicylic acid from aspirin using fluorescence spectroscopic technique and DFT calculations. J Photochem Photobiol B Biol 189:292–297

    Article  CAS  Google Scholar 

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Acknowledgements

Dr. Ranjana Singh want to express her sincere gratitude for getting financial support in terms of CSIR Research Associate ship with file no 09/13 (0846)/2018-EMR-I dated 29/03/2019 by CSIR New Delhi, Govt. of India. Authors are also thankful to Biophysics Laboratory, Department of Physics, B. H. U. Varanasi, India to access the Edinburg FLS 900 fluorescence spectrophotometer under UPE scheme for PL measurement and UV-Visible absorption spectrometer.

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  1. Department of Physics, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

    Ranjana Singh & Ranjan K. Singh

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  1. Ranjana Singh
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  2. Ranjan K. Singh
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Correspondence to Ranjana Singh or Ranjan K. Singh.

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Singh, R., Singh, R.K. Detection of Malachite Green in Water Using Edge Excited Label Free Fluorescent Probe NCQDs. J Fluoresc 30, 1281–1285 (2020). https://doi.org/10.1007/s10895-020-02603-1

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  • DOI: https://doi.org/10.1007/s10895-020-02603-1

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