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Rapid Identification of Dendrobium officinale from Other Species Using 3D Front-Face Fluorescence Technique

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Abstract

Dendrobium officinale is a rare and endangered perennial herb in China, which have been used in preparing Chinese tonic medicine for hundreds of years. The severe shortage of this herb and high price have caused that many similar plants were processed as an adulterant and it became difficult to distinguish genuine D. officinale by traditional authentication methods. A sensitive, convenient, and specific method for rapid identification of D. officinale is urgently needed. In the present study, 3D front-face fluorescence technique merged with Independent Component Analysis was used to get the “pure” independent fluorescence signals. The overall 3D-FFF spectra were decomposed into seven independent components (IC). To distinguish D. officinale from other species, IC1 and IC4 were chosen as fluorescence markers and the fluorescence intensity (FI) value at 340 nm/ 442 nm (excitation /emission wavelength) of IC1and 315 nm/ 468 nm of IC4 were used to build a linear model for identifying D. officinale successfully. Compared with other Dendrobium species, D. officinale showed much higher FI1 and FI4 value which is a direct criterion for identification. Meanwhile, even though the FI values of D. officinale may fluctuate due to the difference of growing conditions, the relation between FI1 and FI4 amazingly always fit the linear model constructed (FI4 = 9.9046 + 0.6119FI1, R2 = 0.9811). The linear model is an important finding and specific for D. officinale. Based on the FI value and the goodness of fit in the linear model, D. officinale can be identified quickly.

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Data Availability

All data generated or analysed during this study are included in this published article and its supplementary information files.

Abbreviations

3D-FFF:

3D front-face fluorescence spectrometry

ICA:

independent component analysis

IC:

independent components

JADE:

Joint Approximate Diagonalization of Eigen matrices

FI:

fluorescence intensity

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Funding

The work was supported by Undergraduate Higher Education Teaching Reform Projects of Guangdong (2017) “Practice and Research of Interactive Teaching Mode-Taking ‘Biochemical Technology Experiment’ as an Example” and Science and Technology Program of Guangdong Province (2020B121201013).

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Authors and Affiliations

  1. College of Life Science & Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Jiaying University, Meisong Road, Jiaying University, Meizhou, 514015, People’s Republic of China

    Heng Xu, Zhi Wei Liu, Liang Zheng Xu, Qiong Feng Yao, Yuan Lan & Jun Chang Liu

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  1. Heng Xu
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  2. Zhi Wei Liu
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  3. Liang Zheng Xu
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  4. Qiong Feng Yao
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Contributions

All members who dedicated to this work are listed as authors, and all authors will take public responsibility for the concept, design, analysis, writing, or revision of the manuscript. Furthermore, each author certifies that this manuscript has not been published in elsewhere before. The specific contributions made by each author have been listed as follows: Heng Xu: conception and design of study, acquisition of data and drafting the manuscript; Zhi Wei Liu, Liang Zheng Xu, Qiong Feng Yao, Yuan Lan, Jun Chang Liu: acquisition of data, analysis and interpretation of data. All authors read and approved the final manuscript.

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Correspondence to Heng Xu.

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Xu, H., Liu, Z.W., Xu, L.Z. et al. Rapid Identification of Dendrobium officinale from Other Species Using 3D Front-Face Fluorescence Technique. J Fluoresc 30, 907–915 (2020). https://doi.org/10.1007/s10895-020-02565-4

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