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Endogenous Fluorescence Dissimilarity Assessment of Four Potential Biomarkers of Early Liver Fibrosis by Preservation Media Effect

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Abstract

Build-up of extracellular matrix in liver fibrosis results in changes on endogenous molecules expression that may be studied through the fluorescence characterization of ex vivo liver samples. To the best of our knowledge, no investigations have provided in-depth evidence and discussion on the changes of the endogenous fluorescence in ex vivo tissue due to the effects of the preservation media. In this work, we contrast and analyze the endogenous fluorescence from tryptophan, vitamin A, hydroxyproline and elastin cross-links potential biomarkers of the liver fibrosis, in in vivo measurements and liver samples preserved on formaldehyde, and two standard preservation media. As it is known, chemical changes in tissue, caused by formaldehyde fixation, alter the endogenous fluorescence spectra. We propose the use of phosphate-buffered saline (PBS), and Iscove’s Modified Dulbecco’s Medium (IMDM) to elude the fluorescence changes. PBS and IMDM showed to maintain the endogenous fluorescence characteristics similar to in vivo conditions. The results of this work point the way for a more reliable assessment of endogenous fluorescence in ex vivo hepatic studies.

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Acknowledgments

Enoch Gutierrez-Herrera and Celia Sánchez-Pérez gratefully acknowledge the Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México (DGAPA UNAM) for financial support through the projects PAPIIT IA103918 and IT100518. The authors also want to express their gratitude to the Laboratorio Universitario de Caracterización Espectroscópica at the Instituto de Ciencias Aplicadas y Tecnología (LUCE-ICAT) and its staff, as well as Nathalie Aleman-Garcia for the valuable support.

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Authors and Affiliations

  1. Instituto de Ciencias Aplicadas y Tecnología (ICAT), Universidad Nacional Autónoma de México (UNAM), Ciudad de México, 04510, México

    Enoch Gutierrez-Herrera, Celia Sánchez-Pérez & Miguel A. Padilla-Castaneda

  2. Unidad de Investigación y Desarrollo Tecnológico, ICAT-Hospital General de México “Dr. Eduardo Liceaga” (HGMEL), Ciudad de México, 06720, México

    Enoch Gutierrez-Herrera & Miguel A. Padilla-Castaneda

  3. Servicio de Cirugía Experimental, HGMEL, Ciudad de México, 06720, México

    Adolfo Perez-Garcia

  4. Wellman Center for Photomedicine, Department of Dermatology, Harvard Medical School, Massachusetts General Hospital, Boston, 02114, MA, USA

    Walfre Franco

  5. Unidad de Farmacología Clínica, HGMEL, Ciudad de México, 06720, México

    Joselín Hernández-Ruiz

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  1. Enoch Gutierrez-Herrera
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  4. Miguel A. Padilla-Castaneda
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  6. Joselín Hernández-Ruiz
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Correspondence to Enoch Gutierrez-Herrera or Joselín Hernández-Ruiz.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted and ethical approval was obtained from the Ethics and Research Committees of the Hospital General de México Dr. Eduardo Liceaga (Reference: DI/16/UME/4/18).

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Gutierrez-Herrera, E., Sánchez-Pérez, C., Perez-Garcia, A. et al. Endogenous Fluorescence Dissimilarity Assessment of Four Potential Biomarkers of Early Liver Fibrosis by Preservation Media Effect. J Fluoresc 30, 249–257 (2020). https://doi.org/10.1007/s10895-019-02484-z

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