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A 3D-Fluorescence Fingerprinting Approach to Detect Physiological Modifications Induced by Pesticide Poisoning in Apis mellifera: A Preliminary Study

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Abstract

The combined use of 3D-fluorescence spectroscopy and independent component analysis using a differential fingerprinting approach has been applied with success to detect physiological effects of dimethoate in honeybees. Biochemical determinations combined with the identification of fluorescence zones that may correspond to proteins, NADH or neurotransmitters/neurohormones (octopamine, dopamine and serotonin) related to the physiological stress caused by the pesticide enabled phenomenological modeling of the physiological response in the honeybee using a simple and rapid method. The signals associated with the fluorophores involved in the response to stress were extracted from the fluorescence spectra using an unsupervised algorithm such as independent component analysis. The signals of different neurotransmitters were isolated on separated factorial components, thus facilitating their biochemical interpretation.

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  1. Dimethoate was used for this study because it is now banned by the European Union. Its marked effects on bee physiology enabled the development of an indirect 3D fluorescence detection methodology.

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Acknowledgements

Our warmest thanks go to members of the Paris Société Centrale d’Apiculture (SCA) for their advice and the monitoring and maintenance of our experimental apiary in the context of a partnership between AgroParisTech and SCA that has been in place since 2013.

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Authors and Affiliations

  1. UMR914 INRA, Physiology of Nutrition and of Eating Behaviour, University of Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France

    Christophe B. Y. Cordella & Alberto Izquierdo-Rodriguez

  2. UMR CNRS 6144 GEPEA, CBAC Laboratory, University of Nantes, Bd Gaston Defferre, 85 000, La Roche sur Yon, France

    Marie-José Durand-Thouand

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  1. Christophe B. Y. Cordella
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Correspondence to Christophe B. Y. Cordella.

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Cordella, C.B.Y., Izquierdo-Rodriguez, A. & Durand-Thouand, MJ. A 3D-Fluorescence Fingerprinting Approach to Detect Physiological Modifications Induced by Pesticide Poisoning in Apis mellifera: A Preliminary Study. J Fluoresc 29, 1475–1485 (2019). https://doi.org/10.1007/s10895-019-02461-6

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