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A Simple Red Emitting “Turn-On” Optical Relay Detector for Al3+ and CN−. Application in the Real Sample and RAW264.7 Cell Imaging

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Abstract

A quantitative and qualitative fluorogenic molecular probe (R) has been designed and synthesized using Rhodamine-B hydrazide and 3,5-dibromo salicylaldehyde. The probe R has been applied for detection of ionic species and probe R turned out to be a prominent distinguisher among divalent and trivalent cations Al3+, Cr3+ and Fe3+ via the fluorometric response in acetonitrile medium. Colorimetric changes were observed for trivalent cations and Cu2+. Among all trivalent cations, Al3+ is possessing relay recognition for CN− even in the presence of other interfering anions with optimum precision. The association constant and the detection limit for R-Al3+ are 4.5 × 106 M−1 and 17.9 nM respectively. On the other hand, R + Al3++CN− exhibits an association constant and detection limit of 5.77 × 105 M−1 and 4.6 nM respectively. The stoichiometry of Al3+ binding with R is found to be 1:1. Quantum efficiency of probe R, R + Al3+ and R + Al3++CN− are found to be 0.2, 0.88 and 0.04 respectively. The receptor showed excellent real-time applicability for commercially available antiperspirant and apple seeds extract. The efficiency of the receptor further extended for fluorescent imaging of Al3+ and CN− in RAW264.7 cells.

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Acknowledgments

The author SN acknowledges the DST-INSPIRE (Department of Science and Technology-India) Fellowship scheme for financial support (IF150881) and authors acknowledge the director of National Institute of Technology-Trichy for providing research infrastructure.

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  1. Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli, India

    Sanay Naha, Muhammed K. Arshad & Sivan Velmathi

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  1. Sanay Naha
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  2. Muhammed K. Arshad
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  3. Sivan Velmathi
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Correspondence to Sivan Velmathi.

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Naha, S., Arshad, M.K. & Velmathi, S. A Simple Red Emitting “Turn-On” Optical Relay Detector for Al3+ and CN−. Application in the Real Sample and RAW264.7 Cell Imaging. J Fluoresc 29, 1401–1410 (2019). https://doi.org/10.1007/s10895-019-02460-7

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