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Analysis of Bisulfite Via a Nitro Derivative of Cyanine-3 (NCy3) in the Microfluidic Channel

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Abstract

NCy3, a derivative of Cyanine 3 with a nitro substituent, showed a high reactivity to bisulfite in aqueous media, instantly leading to ratiometric change of absorption spectra and significant fluorescence quenching. Applied in the microfluidic channel, NCy3 functionalize as a sensitive approach for quantitative detection of bisulfite, particularly for samples with a small volume.

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Acknowledgments

Thanks Dr. Brandon Luedtke for help with the Nikon microscope. Financial support was provided by grants from the National Center for Research Resources (NCRR; 5P20RR016469) and the National Institute for General Medical Science (NIGMS; INBRE-8P20GM103427), a component of the National Institutes of Health (NIH) and Nebraska Research Initiative for the purchase of the Nikon microscope.

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Authors and Affiliations

  1. Department of Chemistry, University of Nebraska-Kearney, Kearney, NE, 68849, USA

    Haley A. Houtwed, Meng Xie, Aatiya Ahmad, Cody D. Masters, Melissa M. Davison, Kristy Kounovsky-Shafer & Haishi Cao

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  1. Haley A. Houtwed
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  2. Meng Xie
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  3. Aatiya Ahmad
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  6. Kristy Kounovsky-Shafer
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  7. Haishi Cao
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Correspondence to Haishi Cao.

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Houtwed, H.A., Xie, M., Ahmad, A. et al. Analysis of Bisulfite Via a Nitro Derivative of Cyanine-3 (NCy3) in the Microfluidic Channel. J Fluoresc 29, 523–529 (2019). https://doi.org/10.1007/s10895-019-02394-0

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  • DOI: https://doi.org/10.1007/s10895-019-02394-0

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