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Multispectroscopic and Computational Investigation of ct-DNA Binding Properties with Hydroxybenzylidene Containing Tetrahydrocarbazole Derivative

Journal of Fluorescence volume 29, pages 101–110 (2019)Cite this article

Abstract

Mode of interaction of a new tetrahydrocarbazole derivative with ct-DNA has been investigated systematically using fluorescence spectroscopy, UV-Vis spectroscopy and circular dichroism spectroscopy. It is concluded that TAH could intercalate into the base pairs of ct-DNA, and the fluorescence quenching by ct-DNA was static quenching type. Beside the multispectroscopic results, computational studies were done. Molecular docking results revealed that the TAH-DNAs complexes might be classified as druggable molecule in drug design. Additionally, DNA binding studies exhibited that TAH complexes have different interaction and orientation abilities to each DNA isomer. Combination of experimental and computational data showed that reported TAH is promising structure and deserves further applications.

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Acknowledgements

This work was supported by the Scientific and Technical Research Council of Turkey (TUBITAK) - Grant No 114Z391.

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Authors and Affiliations

  1. Department of Chemistry, Namık Kemal University, 59030, Tekirdag, Turkey

    Sule Ozkan

  2. Department of Chemistry, Gaziantep University, 27310, Gaziantep, Turkey

    Tugba Taskin-Tok

  3. Department of Chemistry, Hacettepe University, Beytepe, 06800, Ankara, Turkey

    Ayse Uzgoren-Baran

  4. Department of Chemistry, Istanbul Medeniyet University, 34700, Istanbul, Turkey

    Nuriye Akbay

Authors
  1. Sule Ozkan
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  2. Tugba Taskin-Tok
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  4. Nuriye Akbay
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Correspondence to Nuriye Akbay.

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Ozkan, S., Taskin-Tok, T., Uzgoren-Baran, A. et al. Multispectroscopic and Computational Investigation of ct-DNA Binding Properties with Hydroxybenzylidene Containing Tetrahydrocarbazole Derivative. J Fluoresc 29, 101–110 (2019). https://doi.org/10.1007/s10895-018-2314-4

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  • DOI: https://doi.org/10.1007/s10895-018-2314-4

Keywords

  • Fluorescence
  • ct-DNA
  • Tetrahydrocabazoles
  • Stern-Volmer
  • Groove binding