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Synthesis and Studies of Fluorescein Based Derivatives for their Optical Properties, Urease Inhibition and Molecular Docking

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Abstract

Herein, we design and synthesized new fluorescein based derivatives by insitu formation of fluorescein ester and further treated with corresponding hydrazide and amine to yield respective compounds i.e. FB1, FB2, FB3 and FB4. The spectral purity and characterization was done by using IR, NMR and Mass spectroscopies. The synthesized derivatives were examined for their photophysical properties by using variety of organic solvents and results were discussed in details. The structural diversity of synthesized compounds motivate us to evaluate these compounds for urease inhibition. The compound FB3 (IC50 = 0.0456 μM) shows 100 fold more active against Jack bean urease than standard drug thiourea (IC50 = 4.7455 μM). Other synthesized compounds showed potent activity. Free radical percentage scavenging assay further supported the capacity of compounds to urease inhibition. While, molecular docking simulations helps to examine the molecular interactions of active compounds FB1, FB2, FB3 and FB4 within the binding site of urease enzyme.

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Acknowledgments

This work was supported by a grant from Mid-Career Researcher Program (NRF- 2016R1A2B4016552) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP).

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Authors and Affiliations

  1. Department of Chemistry, Kongju National University, Gongju, Chungnam, 32588, Republic of Korea

    Prasad G. Mahajan, Balasaheb D. Vanjare & Ki Hwan Lee

  2. Department of Chemistry, Shivaji University, Kolhapur, Maharashtra, 416004, India

    Nilam C. Dige

  3. Department of Biological Sciences, Kongju National University, Gongju, Chungnam, 32588, Republic of Korea

    Hussain Raza, Mubashir Hassan & Sung-Yum Seo

  4. Division of Bio and Health Sciences, Mokwon University, Seo-gu, Daejeon, 35349, Republic of Korea

    Seong-Karp Hong

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  1. Prasad G. Mahajan
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Correspondence to Ki Hwan Lee.

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Mahajan, P.G., Dige, N.C., Vanjare, B.D. et al. Synthesis and Studies of Fluorescein Based Derivatives for their Optical Properties, Urease Inhibition and Molecular Docking. J Fluoresc 28, 1305–1315 (2018). https://doi.org/10.1007/s10895-018-2291-7

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  • DOI: https://doi.org/10.1007/s10895-018-2291-7

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