Liposomal Form of Tetra(Aryl)Tetracyanoporphyrazine: Physical Properties and Photodynamic Activity In Vitro
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Tetra(aryl)tetracyanoporphyrazines are the promising group of dyes for photodynamic therapy of tumors with unique combination of photosensitizer properties and sensitivity of fluorescence parameters to the environment viscosity. However, in vivo application of such hydrophobic photosensitizers requires using of drug carriers ensuring efficient delivery to the tumor site. The present study is focused on obtaining liposomes loaded with tetrakis(4-benzyloxyphenyl)tetracyanoporphyrazine and examining their properties depending on lipid composition. An efficient loading of the dye and a high long-term stability were proved for the liposomes composed of phosphatidylcholine with cholesterol and phosphatidylglycerol. This can be explained by the presence of negatively charged lipids in the bilayer and, as a consequence, a high value of the surface potential. A high rate of cellular uptake and a strong photoinduced toxicity give the prerequisites for the further use of the liposomal form of the photosensitizer for photodynamic therapy of tumors.
KeywordsPorphyrazine Liposomes Photodynamic therapy Photosensitizer Fluorescent molecular rotor
The work was financially supported by Ministry of Education and Science of the Russian Federation: A.V. Yudintsev and E.L. Guryev are thankful for personal research activity support (grants no. 6.7083.2017/9.10 and no. 20.6515.2017/9.10); N.Yu. Shilyagina is thankful for supporting cellular studies of porphyrazine (contract МК-980.2017.4); I.V. Balalaeva is thankful for supporting chemical synthesis work (government assignment number 6.3099.2017).
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Declaration of Interest
The authors report no conflict of interest.
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