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DNAzyme Based Amplified Biosensor on Ultrasensitive Fluorescence Detection of Pb (II) Ions from Aqueous System

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Abstract

A label -free DNAzyme amplified biosensor is found to be highly selective and sensitive towards fluorescent detection of Pb2+ ions in aqueous media. The DNAzyme complex has designed by the hybridization of the enzyme and substrate strand. In the presence of Pb2+, the DNAzyme activated and cleaved the substrate strand of RNA site (rA) into two oligonucleotide fragments. Further, the free fragment was hybridized with a complementary strand on the surface of MBs. After magnetic separation, SYBER Green I was added and readily intercalate with the dsDNA to gives a bright fluorescence signal with intensity directly proportional to the concentration of Pb2+ions. A detection limit of 5 nM in Pb2+ the detection range 0 to 500 nM was obtained. This label- free fluorescent biosensor has been successfully applied to the determination of environmental water samples. Then results open up the possibility for real-time quantitative detection of Pb2+ with convenient potential applications in the biological and environmental field.

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Authors and Affiliations

  1. Department of Chemistry, SRM University, Kattankulathur, 603 203, Chennai, India

    A. Ravikumar, P. Panneerselvam & K. Radhakrishnan

  2. Environmental Technology Division, School of Industrial Technology, University Sains Malaysia, 11800, Minden, Penang, Malaysia

    Norhashimah Morad

  3. Centre of Bio-Technology, AC Tech, Anna University, Chennai, India

    C. D. Anuradha

  4. Department of Applied Science and Technology, AC Tech, Anna University, Chennai, India

    S. Sivanesan

Authors
  1. A. Ravikumar
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  2. P. Panneerselvam
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  3. K. Radhakrishnan
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  4. Norhashimah Morad
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  5. C. D. Anuradha
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  6. S. Sivanesan
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Correspondence to P. Panneerselvam.

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Ravikumar, A., Panneerselvam, P., Radhakrishnan, K. et al. DNAzyme Based Amplified Biosensor on Ultrasensitive Fluorescence Detection of Pb (II) Ions from Aqueous System. J Fluoresc 27, 2101–2109 (2017). https://doi.org/10.1007/s10895-017-2149-4

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  • DOI: https://doi.org/10.1007/s10895-017-2149-4

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