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Studies on Photocleavage, DNA Binding, Cytotoxicity, and Docking Studies of Ruthenium(II) Mixed Ligand Complexes

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Abstract

This article describes the synthesis and characterization of three new Ru(II) polypyridyl complexes including [Ru(phen)2(dpphz)]2+ (1), [Ru(bpy)2(dpphz)]2+ (2) and [Ru(dmb)2(dpphz)]2+ (3) where dpphz = dipyrido[3,2-a:2′,3′-c] phenazine-11-hydrazide, phen =1,10-phenanthroline, bpy = 2,2′-bipyridine and dmb = 4,4′-dimethyl2,2′-bipyridine. The binding behaviors of these complexes to calf thymus DNA (CT-DNA) were explored by spectroscopic titrations, viscosity measurements. Results suggest that these complexes can bind to CT-DNA through intercalation. However, their binding strength differs from each other; this may be attributed to difference in the ancillary ligand. The cytotoxicity of 1–3 was evaluated by MTT assay; results indicated that all complexes have significant dose dependent cytotoxicity with HeLa tumor cell line. All complexes exhibited efficient photocleavage of pBR322 DNA upon irradiation. The DNA binding ability of 1–3 was also studied by docking the complexes into B-DNA using docking program.

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Acknowledgments

We are grateful to UGC and CSIR New Delhi for financial support and also grateful to CFRD Osmania university and School of chemistry university of Hyderabad, Prof. M.V. Rajashekaran University of Hyderabad and UGC-NRC University of Hyderabad.

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Authors and Affiliations

  1. Department of Chemistry, Osmania University, Hyderabad, India

    Yata Praveen Kumar, A. Srishailam, N. Deepika, V. Ravi Kumar, P. Venkat Reddy & S. Satyanarayana

  2. School of Chemistry, University of Hyderabad, Hyderabad, India

    Yata Praveen Kumar, C. Shobha Devi, A. Srishailam, N. Deepika, V. Ravi Kumar, P. Venkat Reddy, K Nagasuryaprasad, Surya S. Singh, Penumaka Nagababu & S. Satyanarayana

  3. Department of Chemistry, National Dong Hwa University, Hualien, Taiwan, China

    C. Shobha Devi

  4. Department of Biochemistry, Osmania University, Hyderabad, -500007, India

    K Nagasuryaprasad & Surya S. Singh

  5. Inorganic & Physical Chemistry Division, CSIR−Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad, Telangana State, 500007, India

    Penumaka Nagababu

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  1. Yata Praveen Kumar
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Kumar, Y.P., Devi, C.S., Srishailam, A. et al. Studies on Photocleavage, DNA Binding, Cytotoxicity, and Docking Studies of Ruthenium(II) Mixed Ligand Complexes. J Fluoresc 26, 2119–2132 (2016). https://doi.org/10.1007/s10895-016-1908-y

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