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Validation of a Fluorescence Sensor Microtiterplate for Biogenic Amines in Meat and Cheese

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Abstract

An optical sensor microtiterplate for quantitative analysis of the total content of biogenic amines (TAC) in meat and cheese was developed and validated for the first time. In the plate, a chameleon dye (Py-1) is embedded in a polymeric cocktail which is deposited on the bottom of the wells in a common microtiterplate. On reaction with biogenic amines (BAs), the fluorescence of Py-1 at 620 nm rapidly delivers a precise TAC. After 10 min incubation at 25 °C the determination of the TAC in various (real) samples is possible in high-throughput with a standard microplate reader. The optimized fluorescence method was validated for linearity, sensitivity, accuracy, precision (intraday and inter day repeatability) and recovery using histamine (HIS) as a representative BA. The sensor microtiterplate was successfully applied to quantitatively analyze the TAC in 10 real samples of cheese and meat obtained from various Egyptian markets. The TAC of these real samples obtained by the sensor microtiterplate was validated against the contents of BAs obtained by GC-MS at various times of storage. The data of the sensor microtiterplate agreed well with those of GC-MS. This demonstrates that the sensor microtiterplate is a reliable screening tool for the degradation status of food samples.

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Acknowledgments

G.M.K. and M.S.S. thank the German Egyptian Research Fund (GERF) for a grant within the project EGY08/004.

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Authors and Affiliations

  1. Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt

    Gasser M. Khairy, Hassan A. Azab & Sabry A. El-Korashy

  2. Chemistry Department, Faculty of Science and Arts, Aljouf University, P.O. Box # 2014, Skaka-41421, Kingdom of Saudi Arabia

    Gasser M. Khairy

  3. Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040, Regensburg, Germany

    Mark-Steven Steiner & Axel Duerkop

Authors
  1. Gasser M. Khairy
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  2. Hassan A. Azab
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  3. Sabry A. El-Korashy
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  4. Mark-Steven Steiner
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  5. Axel Duerkop
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Correspondence to Axel Duerkop.

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Khairy, G.M., Azab, H.A., El-Korashy, S.A. et al. Validation of a Fluorescence Sensor Microtiterplate for Biogenic Amines in Meat and Cheese. J Fluoresc 26, 1905–1916 (2016). https://doi.org/10.1007/s10895-016-1885-1

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  • DOI: https://doi.org/10.1007/s10895-016-1885-1

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